Targeted Mutagenesis of the Hypophysiotropic Gnrh3 in Zebrafish (Danio rerio) Reveals No Effects on Reproductive Performance
Fig 5
Male and female adult mRNA levels of pituitary gonadotropin genes show no differences between gnrh3+/+ and gnrh3-/- fish.
Pituitary mRNA levels of (A) fshb, (B) lhb, and (C) cga were determined using QPCR. Absolute mRNA levels were normalized to ef1a levels and are presented as mean ± SEM. Differences between genotypes for each sex were determined by a one-tailed, homoscedastic Student t-test and are considered statistically significant when *P ≤ 0.05. gnrh3+/+, grey bars. gnrh3-/-, white bars.