Expanding the Diversity of Mycobacteriophages: Insights into Genome Architecture and Evolution
Figure 5
Evolution of Cluster I genome anatomies.
A. Alignment of genome segments of phages Brujita, Island3, and Che9c. Genes are shown as colored boxes with gene names (a serial number based on that phage) inside the boxes and the pham number indicated above the box with the number of pham members in parentheses. Pairwise nucleotide sequence similarity is shown as colored areas between adjacent genomes, with strength of similarity according to the color spectrum, violet being the most similar, and red the least. B. Alignment of Brujita and Island3 shows that Brujita genes 64 and 66 are related to Island3 genes 65 and 67, respectively, whereas Brujita gene 65 and Island3 gene 66 are distinctly different. The sequences in common are shown bold, and the common genes are shaded dark blue. C. Island3 genes 67 and 68 share a common 60 bp sequence at their 3′ ends. Brujita contains only a single copy of this sequence which represents a recombinant version that matches the upstream part of Island3 67 and the downstream part of Island3 68. Che9c also shares the upstream sequence but is different downstream of gene 75 with sequence discontinuity close to the end of the gene.