Enlarging the Toolbox for Allergen Epitope Definition with an Allergen-Type Model Protein
Figure 5
Amino acids critical forIgE and IgG cross-reactivity in a subgroup of Bet v 1-related proteins.
(A) Binding of serial dilutions of pool serum IgE to surface-coated equimolar amounts of Bet v 1 and Bet v 1_4x (Bet v 1N43I/E45S/N47D/K55A) in ELISA. (B) Inhibition of IgE binding to surface-coated Δ29NCS_5x in the presence of either Bet v 1 or Bet v 1_4x in ELISA. (C) Binding of serial dilutions of monoclonal BV16 to surface-coated equimolar amounts of Bet v 1 and Bet v 1_4x in ELISA. (D) Mediator release induced by recombinant NCS and Bet v 1 variants. Humanized RBL cells were sensitized with a pool of human birch-specific sera. Cross-linking of membrane-bound human IgE by IgE-protein interaction and subsequent release of β-hexosaminidase was determined with serial dilutions of the proteins.