Trichloroethylene-Induced Gene Expression and DNA Methylation Changes in B6C3F1 Mouse Liver
Figure 4
DNA methylation status of the promoter regions of Jun and Myc in the liver from mice exposed to TCE at 0 or 1000 mg/kg b.w. (n = 3).
A, D) Nucleotide sequences of Jun and Myc promoter region fragments (upper strands) and the corresponding bisulphite-converted sequences (lower strands). CpG dinucleotides are numbered and marked in bold. The restriction enzyme cut sites are marked in italic. Primer sequences are underlined. (B, E) Bisulfite sequencing of the Jun and Myc promoter regions. Open and closed circles indicate unmethylated and methylated CpG sites respectively. C) COBRA result of the promoter region of Jun at CpG3,4 (CGCG) by BstUI 171 bp (49/122). F) COBRA result of the promoter region of Myc at CpG4 (TCGA) by TaqI 132 bp (88/43). L: Tiangen DNA ladder; P, positive control by treating mouse genomic DNA with M.SssI. C, control liver samples; T, liver samples treated with TCE at 1000 mg/kg b.w. M methylation; UM, unmethylation.