Prenatal Alcohol Exposure Modifies Glucocorticoid Receptor Subcellular Distribution in the Medial Prefrontal Cortex and Impairs Frontal Cortex-Dependent Learning
Figure 4
Cytosolic (A) and nuclear (B) levels of the mineralocorticoid receptor (MR) in the medial frontal cortex of saccharin (SAC) control and prenatal alcohol exposure (PAE) offspring.
Representative immunoblots are shown below each figure. Subcellular fractions and anti-MR immunoreactivities were prepared as described in the Materials and Methods section. Data are presented as mean immunoreactivity corrected to Coomassie stain ± SEM in the PAE (n = 8–10) and SAC (n = 9–10) mice. Cytosolic MR levels were increased (p<0.05) in the PAE mice, whereas nuclear MR levels were not different in the two groups.