Khellin and Visnagin Differentially Modulate AHR Signaling and Downstream CYP1A Activity in Human Liver Cells
Figure 4
Effect of visnagin and khellin exposure on CYP1A1 protein expression in human primary hepatocytes.
Human hepatocytes were treated with visnagin (VIS; 10 µM and 20 µM), khellin (KHEL; 10 µM and 20 µM), 1 µM 3MC, and/or vehicle (DMSO; 0.1% v/v) for 48 h. The shown western blot panels were obtained using protein lysate from the primary hepatocyte culture LH40. The densitometric analysis presents the results from western blot experiments performed with protein lysates form four different hepatocyte donors (LH42, HEP220624, HEP220586, and LH40; IDV = integrated density volume); (p < 0.05).