Characterization of hepatic macrophages and evaluation of inflammatory response in heme oxygenase-1 deficient mice exposed to scAAV9 vectors
Fig 4
Plasma neutralizing activity and transgene expression in liver macrophages after scAAV9-GFP transduction.
(A) Virus neutralization test in plasma samples collected from WT and HO-1 KO mice 3 days after the 1st administration and 3 days after the 2nd administration of scAAV9-GFP. Flow cytometric analysis of GFP-positive (GFP+) KCs (B) and MDMs (C) in the livers of WT and HO-1 KO mice 3 days after the 1st administration and 3 days after the 2nd administration of scAAV9-GFP. (D) The qPCR assessing the number of vector genomes in the livers of WT and HO-1 KO mice 3 days after the 1st administration and 3 days after the 2nd administration of scAAV9-GFP. Values represent means ± SEM (n = 3-4/group). *p < 0.05 vs. appropriate WT; † p < 0.05 vs. appropriate untreated; # p < 0.05 vs. AAV9-GFP after the 1st administration.