Identification and analysis of key genes involved in methyl salicylate biosynthesis in different birch species
Fig 3
Multiple sequence alignment of SABP2 amino acid sequences constructed from N. tobacco SABP2 (NtSABP2) [7], P. trichocarpa SABP2 (PtSABP2-1, PtSABP2-2) [53] and eight Betula species, including B. lenta (BlSAMT), B. alleghaniensis (BaSAMT), B. grossa (BgSAMT), B. medwediewii (BmSAMT), B. pendula (BpSAMT), B. utilis (BuSAMT), B. nana (BnSAMT) and B. alnoides (BalSAMT).
The blue frames represent the conserved residues, the white characters in red boxes suggest strict identity and the red characters in white boxes specify similarity. The lipase signature sequence of SABP2 is displayed with black frame. The three conserved amino acids forming a catalytic triad, S81, D210 and H238, commonly found in the hydrolase domain, are indicated with a star and are conserved in Betula SABP2 [7], while residues that contact to SA are indicated by arrows [5]. The figure was prepared with ESPript [51].