Next-Generation Sequencing Identifies the Danforth's Short Tail Mouse Mutation as a Retrotransposon Insertion Affecting Ptf1a Expression
Figure 3
Confirmation and mapping of the Sd mutation.
A) Southern analysis and PCR showing the presence of a large DNA insertion at the Sd locus. B) Multiplex PCR from inbred mouse lines showing the mutation is only present in Sd mice, and is not a polymorphism. In this three primer PCR reaction the Sd amplimer is 406 bp, while the WT amplimer is 510 bp. C) Mapping of the Sd mutation which we identified as an ETn (early transposon) in relation to nearby gene/ESTs, figure not to scale.