CELF4 Regulates Translation and Local Abundance of a Vast Set of mRNAs, Including Genes Associated with Regulation of Synaptic Function
Figure 3
CELF4 cosediments with polysomes and large RNA granules.
A. Cortical brain tissue lysates from 4-week old mice were fractionated on 15–55% linear sucrose gradients. Fractions were collected and analyzed by immunoblot with antibodies against CELF4 and ribosomal S6 (S6) protein. Lysates were treated with either EDTA (B) or RNase A (C) in parallel. Sedimentation is show from left to right, with the positions of monosomes (80S), polysomes, and RNA granules indicated.