The Over-Expression of Two Transcription Factors, ABS5/bHLH30 and ABS7/MYB101, Leads to Upwardly Curly Leaves
Figure 3
Expression analysis of ABS5/T5L1.
A. Semi-quantitative RT-PCR analysis of ABS5/T5L1 gene expression in various tissues: roots, two-week-old seedlings, rosette leaves, stems, cauline leaves, siliques and flowers. The expression of Actin2 was used as a control. B. Nuclear localization of ABS5-GFP fusion protein in Arabidopsis leaf protoplasts. Wild type leaf protoplasts were transformed with P35S::GFP or P35S::ABS5-GFP. A single representative protoplast was shown for each transformation. Nuclei were specifically stained by the fluorescent dye Hoechst33342. Hoechst33342, GFP, chlorophyll autofluorescence signals were monitored by fluorescence microscopy. Bright field (BF) images served as controls for protoplast integrity. Bars: 10 µm.