Khellin and Visnagin Differentially Modulate AHR Signaling and Downstream CYP1A Activity in Human Liver Cells
Figure 3
Visnagin and khellin modulate the expression of several AHR target genes in HepG2 cells.
HepG2 cells were pre-treated for 1 h with 20 µM MNF or 0.1% (v/v) DMSO and were subsequently exposed to 10 µM visnagin (VIS) or 10 µM khellin (KHEL). After 16 h, RNA was isolated and reverse transcribed and the expression of CYP1B1, AhRR, PAI-2 and VEGF was measured by qRT-PCR. The data are mean from three independent experiments and were normalized to beta-actin expression. * - value is significantly increased compared to DMSO-treated cells; § - value is significantly reduced in comparison to cells treated with VIS and KHEL, respectively; (p < 0.05).