Genetic Determinants for Pyomelanin Production and Its Protective Effect against Oxidative Stress in Ralstonia solanacearum
Fig 2
Complementation and melanin pigment production in Ralstonia solanacearum strains over time.
(A) Complementation of pyomelanin defective mutants of R. solanaccearum SL341. Cultures of wild type SL341, pyomelanin deficient mutants, and complemented strains in minimal media containing tyrosine after 72 h growth. Each picture from left to right represent wild type, mutant and complemented strain, respectively. (a) Complementation of SL341D (ΔhppD) mutant strain restored its original phenotype. (b) Complementation of SL341S (ΔrpoS) mutant strain. (c) Complementation of SL341G (ΔhrpG) mutant strain. (d) Complementation of SL341R (ΔoxyR) mutant strain, which partially complemented the original phenotype. (B) Melanin pigment production in Ralstonia solanacearum strains over time. Pigment production was examined by measuring the absorbance of cell-free supernatant at 400 nm over time. Vertical bars represent the standard deviation of three biological replicates.