The Over-Expression of Two Transcription Factors, ABS5/bHLH30 and ABS7/MYB101, Leads to Upwardly Curly Leaves
Figure 2
A. Co-segregation analysis of abs5-1D. Genomic DNAs were extracted from 16 F2 progenies from a cross between wild type and abs5-1D. DNA samples were digested with HindIII and separated on an agarose gel before transferred onto a nylon membrane. The DNA gel blot was probed with 32P-labelled BAR gene sequences. Plants with abs5-1D-like phenotypes were marked with asterisks. B. Schematic representation of the T-DNA insertion site in abs5-1D. Open boxes represent genes in the vicinity of the activation tagging T-DNA. Solid lines represent intergenic regions. The orientations of these genes' open reading frames (ORFs) were indicated by arrows. C. Accumulation of At1g68810 transcripts in wild-type and abs5-1D. Equal amounts of total RNA (5 µg) extracted from 2-week-old seedlings were separated on a formaldehyde gel and transferred to a nylon membrane. The blot was hybridized with 32P-labelled full-length At1g68810 cDNA. The ethidium bromide-stained RNA gel served as a loading control. D. Leaf rosettes of representative five-week-old wild type and two independent At1g68810 over-expression lines (OE-1 and OE-2). E. Semi-quantitative RT-PCR analysis of At1g68810 transcripts accumulation in the plants shown in (C).