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Role of Prox1 in the Transforming Ascending Thin Limb of Henle's Loop during Mouse Kidney Development

Fig 2

Prox1 expression during mouse kidney development.

(A-N) Immunofluorescence staining of Prox1 (red) in the kidneys from 18-day-old fetuses (A, E, I) and from 4- (B, F, J), 7- (C, G, K, M) and 21-day-old pups (D, H, L, N). Blue counterstain: DAPI. Scale bars: 20 μm. (O-P) Western blot analysis of Prox1 in the mouse renal medulla at postnatal days 1, 4, 7, 14, 21, 28, and 56. Band intensity was normalized to β-actin. Each bar is the mean of three independent experiments; error lines are SD.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0127429.g002