Distinct Distribution Pattern of Hepatitis B Virus Genotype C and D in Liver Tissue and Serum of Dual Genotype Infected Liver Cirrhosis and Hepatocellular Carcinoma Patients
Figure 2
Quantification of DNA double strand breaks generated after HBV infection.
Huh7 cells (4×104) were transfected with control pTriEX vector and pTriEX-HBV/D, pTriEX-HBV/C plasmids, which contain 1.2× HBV genome, separately in 4 wells glass chamber slide. After 48 hours, (A) cells were fixed, immuno-stained with γ-H2AX antibody and observed under confocal microscope. Scale bar corresponds to 20 µm. (B) The number of foci per cell in ten different fields was counted and average of foci number per cell was plotted. (C) Total protein was isolated and western blot analysis was performed with γ-H2AX antibody. The ratio of band intensity between γ-H2AX and tubulin was determined. The experiment was repeated three times. p<0.05 was considered as significant variation.