Prenatal Alcohol Exposure Modifies Glucocorticoid Receptor Subcellular Distribution in the Medial Prefrontal Cortex and Impairs Frontal Cortex-Dependent Learning
Figure 2
Cytosolic levels of the glucocorticoid receptor (GR), phospho-serine 226 (pS226) GR (left column), phospho-serine 211 (pS211) GR (center column) and phospho-serine 203 (pS203) GR (right column) in the medial frontal cortex of saccharin (SAC) control and prenatal alcohol exposure (PAE) offspring.
Cytosolic fractions were prepared and anti-GR (top row graphs) and anti-phospho-specific GR (middle row graphs) immunoreactivities were determined as described in the Materials and Methods. Anti-total GR and anti-phospho-GR data are presented as the mean (± SEM) immunoreactivities corrected to Coomassie stain. The ratio of the anti-phospho-GR to anti-total GR immunoreactivities (bottom row graphs) was calculated as described in the Materials and Methods. The levels of total GR and all three phospho-GRs, as well as the ratios of all three phospho-GR/total GR, were not different in SAC (n = 7) and PAE (n = 7) mice. Representative Western blots are presented below.