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Overexpression of IRM1 Enhances Resistance to Aphids in Arabidopsis thaliana

Figure 2

Characteristics of IRM1 mutant lines and the effect of this gene on aphid performance.

Phenotype of six week old Columbia-0 (Col-0), IRM1 knock-out mutant (40-KO) and IRM1 overexpressing transgenic lines (G0085, G0088, G0090, G0092); (B) Expression of IRM1 in leaves of Col-0, IRM1 knock out mutant and four independent IRM1 overexpressing transgenic lines. Values are the means (± SD) of three biological replicates; (C) No-choice aphid assays on plants of Col-0, 40-KO and transgenic overexpressing lines. Values are the means (± SD) of 15 biological replicates. Bars marked with different letters are significantly different from each other (ANOVA followed by Tukey tests, P<0.05).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0070914.g002