Folded or Not? Tracking Bet v 1 Conformation in Recombinant Allergen Preparations
Fig 1
Structural integrity of recombinant Bet v 1a and Bet v 1aS112P/R145P.
A) Secondary structure topology of Bet v 1a (pdb: 1BV1). The amino acids S112 (purple) of β-strand 7 and R145 (cyan) of α-helix 3 were exchanged for proline. B) Circular dichroism of rBet v 1a and rBet v 1aS112P/R145P. C) 1H-NMR spectra of 30 μM of rBet v 1a (upper panel), 30 μM rBet v 1aS112P/R145P (middle panel), and the overlay rBet v 1a in red and rBet v 1aS112P/R145P in black (lower panel). 3D-model of Bet v 1a was visualized with PyMOL [54].