Role of Prox1 in the Transforming Ascending Thin Limb of Henle's Loop during Mouse Kidney Development
Fig 1
Expression of Prox1 in the adult mouse kidney.
(A-C) Single immunostaining for Prox1. Scale bars: 200 μm. Co, cortex; G, glomerulus; AA, arcuate artery; LV, lymphatic vessel. (A’) Inset: higer magnification view of rectangle in A. Scale bars: 10 μm. (D–O) Double immunofluorescence staining for Prox1 (red) and LYVE-1 (green), Prox1 (red) and NKCC2 (green), Prox1 (red) and AR (green), and Prox1 (red) and AQP2 (green). Blue counterstain: DAPI. Scale bars: 20 μm. OMCD, outer medullary collecting duct; IMCDi, initial inner medullary collecting duct; IMCDt, terminal inner medullary collecting duct. (P–Q) Western blot analysis of Prox1 in the outer medulla (OM), and initial (IMi), middle (IMm), and terminal (IMt) part of the inner medulla. Band intensity was normalized to β-actin. Each bar is the mean of three independent experiments; error lines are SD.