Summary
Purified plasma membrane vesicles isolated both by aqueous twoPhase methods and by free-flow electrophoresis from homogenates prepared in the presence of 10 mM ascorbate, oxidized external NADH at rates of about 15 nanomoles/min/mg protein. The rate in the isolated vesicles was accelerated, without perceptible lag, 1.5-to 2-fold by 1 to 10 μM auxin (2,4-dichlorophenoxyacetic acid or indole-3-acetic acid). The reaction would be expected to result in acidification of the vesicle interiors and is proposed as a mechanism to account for auxin-induced acidification of cytoplasmin vitro.
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Morré, D.J., Navas, P., Penel, C. et al. Auxin-stimulated NADH oxidase (semidehydroascorbate reductase) of soybean plasma membrane: Role in acidification of cytoplasm?. Protoplasma 133, 195–197 (1986). https://doi.org/10.1007/BF01304635
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DOI: https://doi.org/10.1007/BF01304635