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The complex-type oligosaccharide binding lectin Datura stramonium agglutinin detects type II A muscle fibres in the brachial biceps from man and cat

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Abstract

Complex-type oligosaccharides were detected in the sarcoplasm of muscle fibres from cat and human biceps using lectins and anticarbohydrate antibodies. The lectin Datura stramonium agglutinin strongly stained type II A fibres as identified by myosin ATPase activity after alkaline and acid preincubation. In contrast, all muscle fibres showed a moderate coarse granular staining after incubation with Tetracarpidum conophorum agglutinin and Telfairia occidentalis agglutinin which recognize tri-antennary complex glycans poorly bound by D. stramonium agglutinin. Strong sarcoplasmic staining in all muscle fibres was obtained after incubation with an antibody against branched N-acetyllactosamine structure while an antibody against binary 2←3 sialyllactosamine glycans failed to detect the muscle fibres. Treatment of the muscle sections with sialidase prior to incubation with D. stramonium agglutinin did not influence the lectin staining pattern. Staining of blots from electrophoretically separated muscle proteins obtained byhomogenization, solubilization and centrifugation of small muscle pieces showed D. stramonium agglutinin binding to a number of bands ranging from 200 kDa to 30 kDa. No D. stramonium agglutinin positive bands were observed in blots from separated mitochondrial proteins while blots from sarcoplasmic reticulum separated by electrophoresis stained many bands in the range from 200 kDa to 30 kDA. It may be concluded that all muscle fibres inhuman and cat biceps hold intracellular non-sialylated complex-type oligosaccharides and further, that a specific tri-antennary complex-type glycoform is strongly expressed in type II A fibres as recognized by D. stramonium agglutinin. These results indicate a differential glycosylation of certain myofibrillar-associated proteins in muscle fibre types

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References

  • ABE, K., MCKIBBIN, J. M. & HAKOMORI, S. (1983) The monoclonal antibody directed to difucosylated type 2 chain (Fucα1→2Galβ1→4[Fucα1→3]GlcNAc; Y determinant. J. Biol. Chem. 258, 11793–97.

    PubMed  CAS  Google Scholar 

  • ANIMASHAUN, T., TOGUN, R. A. & HUGHES, C. (1994) Characterization of isolectins In Tetracarpidum conophorumseeds (Nigerian walnut). Glycoconjugate J. 11, 299–303.

    Article  CAS  Google Scholar 

  • CABEZAS-HERRERA, J., MORAL-NARANJO, M. T., CAMPOY, F. J. & VIDAL, C. J. (1994) G4 forms of acetylcholines-terase and butyrylcholinesterase in normal and dystrophic mouse muscle differ in their interaction with Ricinus communisagglutinin. Biochim. Biophys. Acta 1225, 283–8.

    PubMed  CAS  Google Scholar 

  • COLE, R. N. & ZIPSER B. (1994) The carbohydrate binding proteins in the leech: II lactose-binding protein LL35 is located to neural and muscle subsets and all epithelial cells. J. Neurochem. 63, 75–85.

    Article  PubMed  CAS  Google Scholar 

  • COOPER, D. N. & BARONDES, S. H. (1990) Evidence for export of a muscle lectin from cytosol to extracellular matrix and for a novel secretory mechanism. J. Cell. Biol. 110, 1681–91.

    Article  PubMed  CAS  Google Scholar 

  • COOPER, D. N., MASSA, S. M. & BARONDES, S. H. (1991) Endogenous muscle lectin inhibits myoblast adhesion to laminin. J. Cell. Biol. 115, 1437–48.

    Article  PubMed  CAS  Google Scholar 

  • CROWLEY, J. F. & GOLDSTEIN, I. J. (1981) Datura stramoniumlectin: isolation and characterization of the homogeneous lectin. FEBS Lett. 130, 149–52.

    Article  CAS  Google Scholar 

  • DUNN, M. J., SEWRY, C. A. & DUBOWITZ, V. (1982) Cytochemical studies of lectin binding by diseased human muscle. J. Neurol. Sci. 55, 147–59.

    Article  PubMed  CAS  Google Scholar 

  • EGEA, G., GOLDSTEIN, I. J. & ROTH, J. (1989) Light and electron microscopic detection of (3Galβ1,4GlcNAcβ1) sequences in asparagine-linked oligosaccharides with Datura stramoniumlectin. Histochemistry 92, 515–22.

    Article  PubMed  CAS  Google Scholar 

  • ENDO, T., OHBAYASHI, H., HAYASHI, Y., IKEHARA, Y., KOCHIBE, N. & KOBATA, A. (1988) Structural study on the carbohydrate moiety of human placental alkaline phosphatase. J. Biochem. 103, 182–7.

    PubMed  CAS  Google Scholar 

  • FENDERSON B. A., NICHOLS, E. J., CLAUSEN, H. & HAKOMORI, S. (1986) A monoclonal antibody defining a binary N-acetyllactosaminyl structure in lactoisooctaosylceramide (IV6Galβ1→4GlcNAcnLc6): a useful probe for determining differential glycosylation patterns between normal and transformed human fibroblasts. Mol. Immunol. 23, 747–54.

    Article  PubMed  CAS  Google Scholar 

  • KALADAS, P. M., KABAT, E. A., IGLESIAS, J. L., LIS, H. & SHARON, N. (1982) Immunochemical studies on the combining site of the D-Galactose/N-acetyl-D-galactosamine specific lectin from Erythrina cristagalliseeds. Arch. Biochem. Biophys. 217, 624–37.

    Article  PubMed  CAS  Google Scholar 

  • KAWASHIMA, H., SUEYOSHI, S., LI, H., YAMAMOTO, K. & OSAWA, T. (1990) Carbohydrate binding specificities of several poly-N-acetyllactosamine-binding lectins. Glycoconjugate J. 7, 323–34.

    Article  CAS  Google Scholar 

  • KIRKEBY, S. (1996) A monoclonal anticarbohydrate antibody detecting superfast myosin in the masseter muscle. Cell Tissue Res. 283, 85–92.

    Article  PubMed  CAS  Google Scholar 

  • KROG, L. & BOCK, E. (1992) Glycosylation of neural call adhesion molecules of the immunoglobulin super-family. APMIS 100, 53–70.

    Google Scholar 

  • MANDEL, U., CLAUSEN, H., VEDTOFTE, P., SØRENSEN, H. & DABELSTEEN, E. (1988) Sequential expression of carbohydrate antigens with precursor-product relation characterizes cellular maturation in stratified squamous epithelium. J. Oral. Pathol. 17, 506–11.

    Article  PubMed  CAS  Google Scholar 

  • MERKLE, R. K. & CUMMINGS, R. D. (1987) Relationship of the terminal sequences to the length of poly-N-acetyllactosamine chains in asparagine-linked oligosaccharides from the mouse lymphoma cell line BW5147. J. Biol. Chem. 262, 8179–89.

    PubMed  CAS  Google Scholar 

  • NUDELMAN, E. D., MANDEL, U., LEVERY S. B., KAIZU, T. & HAKOMORI, S. (1989) A series of disialogangliosides with binary 2→3 sialosyllactosamine structure, defined by monoclonal antibody NUH2, are oncodevelopmentally regulated antigens. J. Biol. Chem. 264, 18719–25.

    PubMed  CAS  Google Scholar 

  • PENA, S. D. J., GORDON, B. B., KARPATI, G. & CARPENTER, S. (1981) Lectin histochemistry of human skeletal muscle. J. Histochem. Cytochem. 29, 542–6.

    PubMed  CAS  Google Scholar 

  • ROUND, J. M., MATTHEWS, Y. & JONES, D. A. (1981) A quick, simple and reliable histochemical method for ATPase in human muscle preparations. Histochem. J. 12, 707–10.

    Article  Google Scholar 

  • SATO, S., ANIMASHAUN, T. & HUGHES R. C. (1991) Carbohydrate-binding specificity of Tetracarpidum conophorumlectin. J. Biol. Chem. 266, 11485–94.

    PubMed  CAS  Google Scholar 

  • STÅL, P, ERIKSSON, P. O., SCHIAFFINO, S., BUTLER-BROWNE, G. S. & THORNELL, L. E. (1994) Differences in myosin composition between human orofacial, masticatory and limb muscles: enzyme-, immunohisto-and biochemical studies. J. Muscle Res. Cell Motil. 15, 517–34.

    Article  PubMed  Google Scholar 

  • THOMAS, D., LEHMAN, S., KUCHLER, S., MARSCHAL, P. & ZANETTA, J. P. (1994) Differential expression of an endogenous mannose-binding protein R1 during muscle development and regeneration delineating its role in myoblast fusion. Glycobiology 4, 23–38.

    PubMed  CAS  Google Scholar 

  • TOGUN, R. A., ANIMASHAUN, T., BENZIE, C. R. & KAY, J. E. (1988) Purification and characterization of a mitogenic lactose-binding lectin from Tetracarpidum conophorumseeds. In Lectins–Biology, Biochemistry, Clinical Biochemistry vol. 8 (edited by BØG-HANSEN, T. C. & FREED, D. L. J.) pp. 369–72.

  • TOME, F. M., MATSUMURA, K., CHEVALLAY, M., CAMPBELL, K. P. & FARDEAU, M. (1994) Expression of dystrophin-associated glycoproteins during human fetal muscle development: a preliminary immunocyto-chemical study. Neuromuscular Disorders 4, 343–8.

    Article  PubMed  CAS  Google Scholar 

  • VATER, R., HARRIS, J. B., ANDERSON, V. B., ROBERDS, S. L., CAMPBELL, K. P. & CULLEN, M. J. (1995) The expression of dystrophin-associated glycoproteins during skeletal muscle degeneration and regeneration. An immunofluorescence study. J. Neuropathol. Exp. Neurol. 54, 557–000.

    PubMed  CAS  Google Scholar 

  • WU, A. M, KABAT, E. A., GRUEZO, F. G. & PORETZ, R. D. (1981) Immunochemical studies on the reactivities and combining sites of the D-galactopyranose-and 2-acetamido-deoxy-d-galactopyranose-specific lectin purified from Sophora japonicaseeds. Arch. Biochem. Biophys. 209, 191–203.

    Article  PubMed  CAS  Google Scholar 

  • YAMASHITA, K., TOTANI, K., OHKURA, T., TAKASAKI, S., GOLDSTAIN, I. J. & KOBATA, A. (1987) Carbohydrate binding properties of complex-type oligosaccharides on immobilized Datura stramoniumlectin. J. Biol Chem. 262, 1602–7.

    PubMed  CAS  Google Scholar 

  • YOUNG, W. W., PORTOUKALIAN, J., JR. & HAKOMORI, S. (1981) Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-N-glycosyl type II chain. J. Biol Chem. 256, 10967–72.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Oral Function and Physiology, Faculty of Health Sciences, University of Copenhagen, 20 Norre Alle, DK-2200, Copenhagen, Denmark

    SVEND KIRKEBY

  2. National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

    THERESA ANIMASHAUN & R. COLIN HUGHES

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  1. SVEND KIRKEBY
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  2. THERESA ANIMASHAUN
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KIRKEBY, S., ANIMASHAUN, T. & COLIN HUGHES, R. The complex-type oligosaccharide binding lectin Datura stramonium agglutinin detects type II A muscle fibres in the brachial biceps from man and cat. J Muscle Res Cell Motil 18, 31–41 (1997). https://doi.org/10.1023/A:1018624715326

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