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Doubly sensitivity-enhanced 3D TOCSY-HSQC

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Summary

Recently, strategies for double sensitivity enhancement in heteronuclear three-dimensional NMR experiments were introduced (Krishnamurthy, V.V. (1995) J. Magn. Reson., B106, 170–177; Sattler et al. (1995) J. Biomol. NMR, 6, 11–22; Sattler et al. (1995) J. Magn. Reson., B108, 235–242). Since a sensitivity enhancement of a factor 21/2 can be achieved for each indirect dimension, nD spectra can theoretically be enhanced up to a factor of 2((n-1)/2). We propose and analyze a doubly enhanced three-dimensional TOCSY-HSQC sequence. The application of the doubly enhanced three-dimensional {15N, 1H} TOCSY-HSQC sequence is shown for uniformly 13C-/15N- and 15N-labeled samples of the relatively large Azotobacter vinelandii flavodoxin II (179 amino acids). The main factors that contribute to the final signal-to-noise enhancement have been systematically investigated. The sensitivity enhancement obtained for the doubly enhanced TOCSY-HSQC pulse sequence as compared to the standard (unenhanced) version is close to the theoretically expected factor of two.

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References

  • Bax, A. and Pochapsky, S. (1992) J. Magn. Reson., 99, 638–643.

    Google Scholar 

  • Cavanagh, J. and Rance, M. (1990) J. Magn. Reson., 88, 72–85.

    Google Scholar 

  • Grzesiek, S. and Bax, A. (1993) J. Am. Chem. Soc., 115, 12953–12954.

    Google Scholar 

  • Jahnke, W. and Kessler, H. (1994) J. Biomol. NMR., 4, 735–740.

    Google Scholar 

  • Kay, L.E. (1995) Curr. Opin. Struct. Biol., 5, 674–681.

    Google Scholar 

  • Kay, L.E., Keifer, P. and Saarinen, T. (1992) J. Am. Chem. Soc., 114, 10663–10665.

    Google Scholar 

  • Keeler, J. and Neuhaus, D. (1985) J. Magn. Reson., 63, 454–472.

    Google Scholar 

  • Keeler, J., Clowes, R.T., Davis, A. and Laue, E.D. (1994) Methods Enzymol., 239, 145–207.

    Google Scholar 

  • Kontaxis, G., Stonehouse, J., Laue, E.D. and Keeler, J. (1994) J. Magn. Reson., A111, 70–76.

    Google Scholar 

  • Krishnamurthy, V.V. (1995) J. Magn. Reson., A106, 170–177.

    Google Scholar 

  • Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989) J. Magn. Reson., 85, 393–399.

    Google Scholar 

  • PalmerIII, A.G., Cavanagh, J., Wright, P.E. and Rance, M. (1991) J. Magn. Reson., 93, 151–170.

    Google Scholar 

  • Sattler, M., Schwendinger, M., Schleucher, J. and Griesinger, C. (1995a) J. Biomol. NMR, 6, 11–22.

    Google Scholar 

  • Sattler, M., Schmidt, P., Schleucher, J., Schledetzky, O., Glaser, S.J. and Griesinger, C. (1995b) J. Magn. Reson., B108, 235–242.

    Google Scholar 

  • Schleucher, J., Schwendinger, M., Sattler, M., Schmidt, P., Schledetzky, O., Glaser, S.J., Sörensen, O.W. and Griesinger, C. (1994) J. Biomol. NMR, 4, 301–306.

    Google Scholar 

  • Shaka, A.J., Barker, P.B. and Freeman, R. (1985) J. Magn. Reson., 64, 547–558.

    Google Scholar 

  • Shaka, A.J., Lee, C.J. and Pines, A. (1988) J. Magn. Reson., 77, 274–293.

    Google Scholar 

  • Stonehouse, J., Shaw, G.L., Keeler, J. and Laue, E.D. (1994) J. Magn. Reson., A107, 178–184.

    Google Scholar 

  • Stonehouse, J., Clowes, T.C., Shaw, G.L., Keeler, J. and Laue, E.D. (1995) J. Biomol. NMR, 5, 226–232.

    Google Scholar 

  • Van deVen, F.J.M. (1995) Multi-dimensional NMR in Liquids, VCH Publishers, Cambridge, U.K., pp. 71–72.

    Google Scholar 

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Authors and Affiliations

  1. SON/NWO National HF-NMR Facility, Nijmegen SON Research Center for Molecular Design, Structure and Synthesis, Toernooiveld, 6525 ED, Nijmegen, The Netherlands

    Sybren S. Wijmenga

  2. Department of Biochemistry, Wageningen Agricultural University, Dreyenlaan 3, 6703 HA, Wageningen, The Netherlands

    Carlo P. M. van Mierlo & Elles Steensma

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  1. Sybren S. Wijmenga
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  2. Carlo P. M. van Mierlo
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  3. Elles Steensma
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Wijmenga, S.S., van Mierlo, C.P.M. & Steensma, E. Doubly sensitivity-enhanced 3D TOCSY-HSQC. J Biomol NMR 8, 319–330 (1996). https://doi.org/10.1007/BF00410330

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  • DOI: https://doi.org/10.1007/BF00410330

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