Skip to main content
SpringerLink
Log in

Chemical characterization of cell-wall polysaccharides from tank-cultivated and wild plants ofDelesseria sanguinea (Hudson) Lamouroux (Ceramiales, delesseriaceae): culture patterns and potent anticoagulant activity

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

Tank cultivation ofDelesseria sanguinea was investigated in order to manipulate conditions for vegetative growth and to provide biomass for the analysis of cell wall polysaccharides. Seasonality is subject to short-day photoperiodic control. Night-break or long-day conditions prevented fertility in tetrasporophytes and gametophytes and triggered outgrowth of new blades. Long-day illuminations allowed a 1% daily growth rate. Seawater temperature below 13 °C was necessary for inducing formation of new blades. Both wild and cultivated ofD. sanguinea plants contained a non gelling sulfated heteropolysaccharide composed of a galactosyl backbone branched with xylosyl residues. The hot water extract at neutral pH displayed the highest anticoagulant activity (5 μg ml-1 polysaccharide concentration in APTT clotting assay). No obvious differences were found in polysaccharide chemical composition and properties between gametophytes and sporophytes or between cultivated and wild plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

APTT:

activated partial thromboplastin time

TFA:

trifluroacetic acid

PPP:

platelets poor plasma

References

  • Albersheim P, Nevius DJ, English PD, Karr A (1967) A method for the analysis of sugars in plant cell wall polysaccharide by gas liquid chromatography. Carbohydr. Res. 5: 340–345.

    Article  CAS  Google Scholar 

  • Anderson W, Duncan JGC (1965) The anticoagulant activity of carrageenan. J. Pharm. Pharmacol. 17: 647–654.

    PubMed  CAS  Google Scholar 

  • Bellion C, Brigand G, Prome JC, Welti D, Bociek S (1983) Identification et catactérisation des précurseurs biologiques des carraghénanes par spectroscopie de R.M.N.-13C. Carbohydr. Res. 119: 31–48.

    Article  CAS  Google Scholar 

  • Blumenkrantz NG, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal. Biochem. 54: 484–489.

    Article  PubMed  CAS  Google Scholar 

  • Bosso C, Heyraud A, Patron L (1991) Oligosaccharide characterizations by fast atom bombardement mass spectrometry. Fragmentation study in relation with the substituent at the reducing end. Org. Mass Spectrometry 26: 321–334.

    Article  CAS  Google Scholar 

  • Chapman ARO, Craigie JS (1977) Seasonal growth inLaminaria longicruris: relations with dissolved inorganic nutrients and internal reserves of nitrogen. Mar. Biol. 40: 197–205.

    Article  CAS  Google Scholar 

  • Chapman ARO, Craigie JS (1977) Seasonal growth inLaminaria longicruris: relations with reserves carbohydrate storage and production. Mar. Biol. 46: 209–213.

    Article  Google Scholar 

  • Chargaff E, Bancroft FW, Stanley-Brown M (1936) Studies on the chemistry of blood coagulation II. On the inhibition of blood clotting by substances of high molecular weight. J. biol. Chem. 115: 155–161.

    CAS  Google Scholar 

  • Chevolot L, Cochard JC, Yvin JC (1991) Chemical induction of larval metamorphosis ofPecten maximus with a note on the nature of naturally occurring triggering substances. Mar. Ecol. Progr. Ser. 74: 83–89.

    CAS  Google Scholar 

  • Chopin T, Hanisak MD, Koehn FE, Mollion J, Moreau S (1990) Studies on carrageenans and effects of seawater phosphorus concentration on carrageenan content and growth ofAgardhiella subulata (C. Agardh) Kraft and Wynne (Rhodophyceae, Solieriaceae). J. appl. Phycol. 2: 3–16.

    Article  Google Scholar 

  • Church FC, Meade JB, Treanor RE, Whinna HC (1989) Antithrombin activity of fucoidan. J. biol. Chem. 264: 3618–3623.

    PubMed  CAS  Google Scholar 

  • Craigie JS, Wen ZC (1984) Effects of temperature and tissue age on gel strength and composition of agar fromGracilaria tikvahiae (Rhodophyceae). Can. J. Bot. 62: 1662–1670.

    Article  Google Scholar 

  • Dais P, Perlin AS (1982) High field13C-NMR spectroscopy of β-D glucans, amylopectin and glycogen. Carbohydr. Res. 100: 103–116.

    Article  CAS  Google Scholar 

  • Dauvin JC, Joncourt M, Birrien JL (1991) Température et salinité de l'eau de mer au large de Roscoff de 1988 à 1990. Cah. Biol. mar. 32: 545–550.

    Google Scholar 

  • Deacon-Smith RA, Lee-Potter JP, Rogers DJ (1985) Anticoagulant activity in extracts of British marine algae. Bot. mar. 28: 333–338.

    Article  Google Scholar 

  • Elsner H, Brosner W, Burgel E (1937) Uber das vorkomen von hochwirksamen die blutgerinnung hemmenden stofen in rotalgen. Ztschr. Physiol. Chem. 246: 244–247.

    CAS  Google Scholar 

  • Fortes MD, Lüning K (1980) Growth rates of North Sea macroalgae in relation to temperature, irradiance and photoperiod. Helgoländer Meeresunters, 34: 15–29.

    Article  Google Scholar 

  • Furneaux RH, Stevenson TT (1990) The xylogalactan sulfate fromChondria macrocarpa (Ceramiales, Rhodophyta). Hydrobiologia 204/205: 615–620.

    Article  Google Scholar 

  • Grauffel V, Kloareg B, Mabeau S, Durand P, Josefonvicz J (1989) New natural polysaccharides with potent antithrombic activity: fucans from brown algae. Biomaterials 10: 363–368.

    Article  PubMed  CAS  Google Scholar 

  • Hakomori S (1964) Rapid permethylation of glycolipids and polysaccharides catalysed by methyl sulfinyl carbanion in dimethyl sulfoxide. J. Biochem. (Tokyo) 55: 205–208.

    CAS  Google Scholar 

  • Indergaard M, Skjak-Braek G, Jensen A (1990) Studies on the influence of nutrients on the composition and structure of alginate inLaminaria saccharina (L.) Lamour. (Laminariales, Phaeophyceae). Bot mar. 33: 277–288.

    CAS  Google Scholar 

  • Jackson SG, McCandless EL (1978) Simple, rapid, turbidimetric determination of inorganic sulfate and on proteins. Analyt. Biochem. 90: 802–808.

    Article  PubMed  CAS  Google Scholar 

  • Kain JM (1984) Seasonal growth of two subtidal species of Rhodophyta off the Isle of Man. J. exp. mar. Biol. Ecol. 82: 207–220.

    Article  Google Scholar 

  • Kain JM (1987) Photoperiod and temperature as triggers in the seasonality ofDelesseria sanguinea. Helgoländer Meeresunters. 41: 355–370.

    Article  Google Scholar 

  • Kain JM (1989) The seasons in the subtidal. Br. phycol. J. 24: 203–215.

    Google Scholar 

  • Kindness G, Long WF, Williamson FB (1979) Enhancement of antithrombin III activity by carrageenans. Thrombosis Research 15: 49–60.

    Article  PubMed  CAS  Google Scholar 

  • Kindness G, Long WF, Williamson FB (1980) Anticoagulant effects of sulfated polysaccharides in normal and antithrombin III-deficient plasmas. Br. J. Pharmac. 69: 675–677.

    CAS  Google Scholar 

  • Knutsen SH, Grasdalen H (1987) Characterization of water-extractable polysaccharides from NorwegianFurcellaria lumbricalis (Huds.) Lamour. (Gigartinales, Rhodophyceae) by IR and NMR spectroscopy. Bot. mar. 30: 497–505.

    CAS  Google Scholar 

  • Lahaye M, Rochas C, Yaphe W (1986) A new procedure for determining the heterogeneity of agar polymers in the cell walls ofGracilaria spp. (Gracilariaceae, Rhodophyta). Can. J. Bot. 64: 579–585.

    Article  CAS  Google Scholar 

  • Purdie T, Irvine JC (1903) The alkylation of sugars. J. Chem. Soc. 83: 1021–1037.

    CAS  Google Scholar 

  • Rimington C (1931) The carbohydrate complex of serum proteins. II. Improved method for isolation and redetermination of structure. Isolation of glucosaminodimannose from protein of ox blood. Biochem. J. 25: 1062–1071.

    PubMed  CAS  Google Scholar 

  • Rogers DJ, Jurd KM, Blunden G, Paoletti S, Zanetti F (1990) Anticoagulant activity of a proteoglycan in extracts ofCodium fragile spp.atlanticum. J. appl. Phycol. 2: 357–361.

    Article  CAS  Google Scholar 

  • Saeman JF, Moore WE, Mitchell RL, Millet MA (1954) Techniques for the determination of pulp constituants by quantitative paper chromatography. Tappy 37: 336–343.

    CAS  Google Scholar 

  • Sawardeker JS, Sloneker JH, Jeanes A (1965) Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromoatography. Anal. Chem. 37: 1602–1604.

    Article  CAS  Google Scholar 

  • Scott JE (1960) Aliphatic ammonium salts in the assay of acidic polysaccharides from tissue. Meth. Biochem. Analysis 8: 145–197.

    CAS  Google Scholar 

  • Springer GF, Wurzel HA, McNeal GM, Ansell NJ, Doughty MF (1957) Isolation of anticoagulant fractions from crude fucoidin. Proc. Soc. Exp. Biol. Med. 94: 404–409.

    PubMed  CAS  Google Scholar 

  • Turner RD, Evans LV (1986) Structural and physiological aspects of translocation in the red algaDelesseria. Br. phycol. J. 21: 338.

    Google Scholar 

  • Usov AI, Ivanova EG, Shahkov AS (1983) Polysaccharides of algae XXXIII: Isolation and13C-NMR spectral study of some new gel-forming polysaccharides from Japan Sea red seaweeds. Bot. mar. 26: 285–294.

    Article  CAS  Google Scholar 

  • Yaphe WE, Arsenault GP (1965) Improved resorcinol reagent for the determination of fructose and 3,6-anhydrogalactose in polysaccharides. Analyt. Biochem. 13: 143–148.

    Article  CAS  Google Scholar 

  • Yvin JC (1984) Thèse de Docteur Ingénieur, U.B.O. Brest, 198 pp.

  • Yvin JC, Chevolot L, Chevolot-Magueur AM, Cochard JC (1985) First isolation of jacaranone from an alga,Delesseria sanguinea. A metamorphosis inducer ofPecten larvae. J. Nat. prods 48: 814–816.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre d'Etudes d'Océanographie et de Biologie Marine, CNRS UPR 4601, BP 74, 29682, Roscoff Cedex, France

    Philippe Potin, Pascale Patier & Bernard Kloareg

  2. Laboratoire de Physiologie Végétale, Faculté des Sciences, 29287, Brest Cedex, France

    Jean-Yves Floc'h

  3. Centre d'Etudes sur les macromolécules Végétales, CERMAV-CNRS, associé à l'Université Joseph Fourier de Grenoble, BP 53X, 38041, Grenoble, France

    Pascale Patier & Cyrille Rochas

  4. Laboratoires Goëmar, Z.A.C. La Madeleine, BP 55, 35413, Saint Malo Cedex, France

    Jean-Claude Yvin

Authors
  1. Philippe Potin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pascale Patier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jean-Yves Floc'h
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jean-Claude Yvin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cyrille Rochas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bernard Kloareg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Potin, P., Patier, P., Floc'h, JY. et al. Chemical characterization of cell-wall polysaccharides from tank-cultivated and wild plants ofDelesseria sanguinea (Hudson) Lamouroux (Ceramiales, delesseriaceae): culture patterns and potent anticoagulant activity. J Appl Phycol 4, 119–128 (1992). https://doi.org/10.1007/BF02442460

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02442460

Key words

Search

Navigation