Abstract
This report illustrates the complex enzymology of the multisubstrate protein phosphate that reverses most of the cyclic AMP-mediated protein phosphorylation reactions that regulate glycogen metabolism. The activity of the protein phosphatase is controlled in a dual way: it interconverts between an active and an inactive form, while the expression of its activity can furthermore be prevented by a heat-stable protein (inhibitor-1). The interconversion of the mutisubstrate protein phosphatase is made possible by the presence of a modulator protein, which constitutes the enzyme's regulatory subunit, and by the action of an activating protein, the kinase FA, which is responsible for the transition of the enzyme's catalytic subunit into its active conformation.
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Brandt, H., Killilea, S. D., and Lee, E. Y. C. (1974).Biochem. Biophys. Res. Commun. 61, 598–604.
Brandt, H., Capulong, Z. L., and Lee, E. Y. C. (1975a).J. Biol. Chem. 250, 8038–8044.
Brandt, H., Liee, E. Y. C., and Killilea, S. D. (1975b).Biochem. Biophys. Res. Commun. 63, 950–956.
Detwiler, T. C., Gratecos, D., and Fischer, E. H. (1977).Biochemistry 16, 4818–4823.
Foulkes, J. G., and Cohen, P. (1980).Eur. J. Biochem. 105, 195–203.
Foulkes, J. G., Jefferson, L. S., and Cohen, P. (1980).FEBS Lett. 112, 21–24.
Foulkes, J. G., Cohen, P., Strada, S., Everson, W. V., and Jefferson, L. S. (1982).J. Biol. Chem. 257, 12493–12496.
Goris, J., Defreyn, G., and Merlevede, W. (1977).Biochimie 59, 171–178.
Goris, J., Defreyn, G., and Merlevede, W. (1979).FEBS Lett. 99, 279–282.
Goris, J., Camps, T., Defreyn, G., and Merlevede, W. (1981).FEBS Lett. 134, 189–193.
Gragecos, D., Detwiler, T. C., Hurd, S., and Fischer, E. H. (1977).Biochemistry 16, 4812–4817.
Hemmings, B., Resink, T. J., and Cohen, P. (1982).FEBS Lett 15, 319–324.
Huang, F. L., and Glinsmann, W. H. (1975).Proc. Natl. Acad. Sci. 72, 3004–3008.
Huang, F. L., and Glinsmann, W. H. (1976).Eur. J. Biochem. 70, 419–426.
Imoaka, T., Imazu, M., Usui, H., Kinohara, N., and Takeda, M. (1983).J. Biol. Chem. 258, 1526–1535.
Ingebritsen, T. S., Foulkes, J. G., and Cohen, P. (1983).Eur. J. Biochem. 132, 263–274.
Jurgensen, S., Shacter, E., and Huang, C. Y., Chock, P. B., Yang, S. D., Vandenheede, J. R., and Merlevede, W. (1983).J. Biol. Chem.,259, 5864–5870.
Kato, K., and Sato, S. (1974).Biochim. Biophys. Acta 358, 299–307.
Khandelwal, R. L., and Zinman, S. M. (1978).J. Biol. Chem. 253, 560–565.
Khandelwal, R. L., Vandenheede, J. R., and Krebs, E. G. (1976).J. Biol. Chem. 251, 4850–4858.
Khatra, B. S., Chiasson, J.-L., Shikima, H., Exton, J. H., and Soderling, T. R. (1980).FEBS Lett. 114, 253–256.
Killiea, S. D., Brandt, H., Lee, E. Y. C., and Whelan, W. J. (1976).J. Biol. Chem. 251, 2363–2368.
Killilea, S. D., Aylward, J. H., Mellgren, R. L., and Lee, E. Y. C. (1978).Arch. Biochem. Biophys. 191, 638–646.
Lee, E. Y. C., Brandt, H., Capulong, Z. L., and Killilea, S. D. (1976).Adv. Enz. Regul. 14, 467–490.
Lee, E. Y. C., Mellgren, R. L., Killilea, S. D., and Aylward, J. H. (1978). InRegulatory Mechanisms of Carbohydrate Metabolism (Esmann, V., ed.), Pergamon Press, New York, pp. 327–346.
Lee, E. Y. C., Silberman, S. R., Ganapathi, M. K., Petrovic, S., and Paris, H. (1980).Adv. Cycl. Nucl. Res. 13, 95–131.
Mellgren, R. L., Aylward, J. H., Killilea, S. D., and Lee, E. Y. C. (1979).J. Biol. Chem. 254, 648–652.
Merlevede, W., and Riley, G. A. (1966).J. Biol. Chem. 241, 3517–3524.
Merlevede, W., Vandenheede J. R., Goris, J., and Yang, S. D. (1984).Curr. Top. Cell. Regul. 23, 177–215.
Nimmo, G. A., and Cohen, P. (1978a).Eur. J. Biochem. 87, 341–351.
Nimmo, G. A., and Cohen, P. (1978b).Eur. J. Biochem. 87, 353–365.
Pato, M. D., and Adelstein, R. S. (1980).J. Biol. Chem. 255, 6535–6538.
Pato, M. D., Adelstein, R. S., Crouch, D., Safer, B., Ingebritsen, T. S., and Cohen, P. (1983).Eur. J. Biochem. 132, 283–287.
Resink, T. J., Hemmings, B. A., Tung, H. Y., and Cohen, P. (1983).Eur. J. Biochem. 133, 455–461.
Rouch, P. J. (1981).Curr. Top. Cell. Regul. 20, 45–105.
Stewart, A. A., Hemmings, B. A., Cohen, P., Goris, J., and Merlevede, W. (1981),Eur. J. Biochem. 115, 197–205.
Stewart, A., Ingebritsen, T. S., Manalan, A., Klee, C. B., and Cohen, P. (1982).FEBS Lett. 137, 80–84.
Tamura, S., Kikuchi, K., Hiraga, A., Kikuchi, H., Hosokawa, M., and Tsuiki, S. (1978).Biochim. Biophys. Acta 524, 349–356.
Tao, S.-H., Huang, F. L., Lynch, A., and Glinsmann, W. H. (1978).Biochem. J. 176, 347–350.
Vandenheede, J. R., Yang, S.-D., and Goris, J., and Merlevede, W. (1980).J. Biol. Chem. 255, 11768–11774.
Vandenheede, J. R., Yang, S.-D., and Merlevede, W., (1981a).J. Biol. Chem. 256, 5894–5900.
Vandenheede, J. R., Goris, J., Yang, S.-D., and Camps, T., and Merlevede, W. (1981b).FEBS Lett. 127, 1–3.
Vandenheede, J. R., Yang, S.-D., and Merlevede, W. (1983).Biochem. Biophys. Res. Commun. 115, 871–877.
Yang, S.-D., Vandenheede, J. R., Goris, J., and Merlevede, W. (1980a).FEBS Lett. 111, 201–204.
Yang, S.-D., Vandenheede, J. R., Goris, J., and Merlevede, W. (1980b).J. Biol. Chem. 255, 11759–11767.
Yang, S.-D., Vandenheede, J. R., and Merlevede, W. (1981a).J. Biol. Chem. 256, 10231–10234.
Yang, S.-D., Vandenheede, J. R., and Merlevede, W. (1981b).FEBS Lett. 132, 293–295.
Yang, S.-D., Tallant, E. A., and Cheung, W. Y. (1982).Biochem. Biophys. Res. Commun. 106, 1419–1425.
Yang, S.-D., and Vandenheede, J. R., and Merlevede, W. (1983).Biochem. Biophys. Res. Commun. 113, 439–445.
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Vandenheede, J.R., Yang, SD. & Merlevede, W. Protein phosphatase interconversions. J Protein Chem 3, 157–166 (1984). https://doi.org/10.1007/BF01040497
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DOI: https://doi.org/10.1007/BF01040497