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  • 1
    Online-Ressource
    Online-Ressource
    New York : NYU Press
    Schlagwort(e): Electronic books
    Beschreibung / Inhaltsverzeichnis: Love and Money argues that we can't understand contemporary queer cultures without looking through the lens of social class. Resisting old divisions between culture and economy, identity and privilege, left and queer, recognition and redistribution, Love and Money offers supple approaches to capturing class experience and class form in and around queerness. Contrary to familiar dismissals, not every queer television or movie character is like Will Truman on Will and Grace -rich, white, healthy, professional, detached from politics, community, and sex. Through ethnographic encounters with rea
    Materialart: Online-Ressource
    Seiten: Online-Ressource (214 p)
    Ausgabe: Online-Ausg. 2013 Electronic reproduction; Available via World Wide Web
    ISBN: 9780814790571
    Serie: Critical cultural communication
    DDC: 306.76/6
    Sprache: Englisch
    Anmerkung: Description based upon print version of record , Cover; Contents; Acknowledgments; Introduction: Love and Money, Queerness and Class; 1. The Class Character of Boys Don't Cry; 2. Queer Visibility and Social Class; 3. Every Queer Thing We Know; 4. Recognition: Queers, Class, and Dorothy Allison; 5. Queer Relay; 6. Plausible Optimism; Conclusion: A Cultural Politics of Love and Solidarity; Notes; References; Films; Television Programs; Index; A; B; C; D; E; F; G; H; I; J; K; L; M; N; O; P; Q; R; S; T; U; V; W; X; Z; About the Author; , Electronic reproduction; Available via World Wide Web
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Publikationsdatum: 2015-10-03
    Beschreibung: Background: Setaria viridis has emerged as a model species for the larger C 4 grasses. Here the cellulose synthase (CesA) superfamily has been defined, with an emphasis on the amounts and distribution of (1,3;1,4)-β-glucan, a cell wall polysaccharide that is characteristic of the grasses and is of considerable value for human health. Methods: Orthologous relationship of the CesA and Poales-specific cellulose synthase-like (Csl) genes among Setaria italica (Si), Sorghum bicolor (Sb), Oryza sativa (Os), Brachypodium distachyon (Bradi) and Hordeum vulgare (Hv) were compared using bioinformatics analysis. Transcription profiling of Csl gene families, which are involved in (1,3;1,4)-β-glucan synthesis, was performed using real-time quantitative PCR (Q-PCR). The amount of (1,3;1,4)-β-glucan was measured using a modified Megazyme assay. The fine structures of the (1,3;1,4)-β-glucan, as denoted by the ratio of cellotriosyl to cellotetraosyl residues (DP3:DP4 ratio) was assessed by chromatography (HPLC and HPAEC-PAD). The distribution and deposition of the MLG was examined using the specific antibody BG-1 and captured using fluorescence and transmission electron microscopy (TEM). Results: The cellulose synthase gene superfamily contains 13 CesA and 35 Csl genes in Setaria. Transcript profiling of CslF, CslH and CslJ gene families across a vegetative tissue series indicated that SvCslF6 transcripts were the most abundant relative to all other Csl transcripts. The amounts of (1,3;1,4)-β-glucan in Setaria vegetative tissues ranged from 0.2% to 2.9% w/w with much smaller amounts in developing grain (0.003% to 0.013% w/w). In general, the amount of (1,3;1,4)-β-glucan was greater in younger than in older tissues. The DP3:DP4 ratios varied between tissue types and across developmental stages, and ranged from 2.4 to 3.0:1. The DP3:DP4 ratios in developing grain ranged from 2.5 to 2.8:1. Micrographs revealing the distribution of (1,3;1,4)-β-glucan in walls of different cell types and the data were consistent with the quantitative (1,3;1,4)-β-glucan assays. Conclusion: The characteristics of the cellulose synthase gene superfamily and the accumulation and distribution of (1,3;1,4)-β-glucans in Setaria are similar to those in other C 4 grasses, including sorghum. This suggests that Setaria is a suitable model plant for cell wall polysaccharide biology in C 4 grasses.
    Digitale ISSN: 1471-2229
    Thema: Biologie
    Publiziert von BioMed Central
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