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  • OceanRep  (6)
  • 1
    Publication Date: 2022-06-08
    Description: In anoxic sediment from Kiel Bight short-term incorporation of tritiated thymidine (tdr) into DNA was partly reduced by molybdate, a fairly specific inhibitor of sulfate-reducing bacteria (SRB). Preliminary incorporation experiments with cultures of SRB, however, failed to provide substantial clues if and how SRB account for incorporation of tdr in anoxic sediments. One single positive response in four strains tested was by two orders of magnitude too low to compare with aerobic heterotrophic bacteria.
    Type: Article , NonPeerReviewed
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  • 2
    Publication Date: 2022-06-08
    Description: Deposit-feeding benthic macrofauna withdraw substantial amounts of bacterial biomass from marine sediments. On the other hand, burrowing macro-invertebrates can mediate epizoic and perizoic enrichment of bacteria by tapping or "conditioning" energy carriers (for heterotrophic and chemolithotrophic bacteria) that are stored in the sediment. Selected examples show how this applies particularly to reduced sediments.
    Type: Article , NonPeerReviewed
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  • 3
    Publication Date: 2022-06-07
    Description: The priapulid worm Halicryptus spinulosus which occurs in sulfide-rich mud of Kiel Bight is covered with a dense epizoic biofilm. Several types of filamentous microorganisms can be distinguished by SEM confirming previous conclusions based on TEM investigations. The most conspicuous forms are firmly attached filaments resembling Thiothrix that accumulate at the cuticular setae. Enrichment experiments indicate the presence of sulfide-oxidizing bacteria. A vector hypothesis is postulated which implies that the redox requirements of the (S)­-oxidizing constituents of the biofilm are matched by the worm's frequent moves between oxic and anoxic layers of the sediment.
    Type: Article , NonPeerReviewed
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  • 4
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    Institut für Meereskunde
    Publication Date: 2022-06-07
    Description: Starvation affects marine bacteria also under anaerobic conditions. Some basic data obtained for anaerobic starvation survival of a fermentative and a sulfaterespiring strain indicate substantial differences. The fermentative strain, Listonella (= Vibrio) anguillarum, responded to, nutrient depletion with rapid reduction of their cell size (dwarfing) and decline of viable cell counts by three orders of magnitude. The sulfate-respiring Desulfovibrio vulgaris showed only minor reductions of the cell sizes and no loss of viability. Whereas a drastic decline of cellular protein concentrations in this strain indicated strong endogeneous respiration, starved cells of the fermenting Vibrio sp. showed increasing levels of protein after an initial decrease.
    Type: Article , NonPeerReviewed
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  • 5
    Publication Date: 2022-06-03
    Description: For brackish water bodies at the North Sea coast which harbor red tide algal blooms during the summer months, phytoplankton densities and chlorophyll a data were compared with bacterial counts including plate counts of TCBS-agar-selected vibrios. Concentrations of chlorophyll a, the ratios of total viable counts on ZoBell agar to acridine orange direct counts (CFU/ AODC), and vibrio counts on TCBS-agar followed largely the same trends suggesting a strong linkage between phytoplankton biomass and heterotrophic cacteria. Enrichment experiments based on macroalgal extracts confirmed the extremely copiotrophic nature of TCBS-selected vibrios. Vibrio counts peaked during the bloom of a Cryptomonas sp. that was presumably grazed upon by a ciliate, Strombidium sp.. On the other hand, there were no indications of an analogous coincidence during mass developments of the potentially toxic dinoflagellate Glenodinium foliaceum.
    Type: Article , NonPeerReviewed
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  • 6
    Publication Date: 2022-06-03
    Description: A direct microscopic method based on the response of bacterial cells to inhibition of DNA synthesis by nalidixic acid in the presence of growth-supporting yeast extract and designed to determine the number of viable bacteria, was tested in marine sediments bioturbated by the deposit-feeding polychaete Arenicola marina. The number of responsive cells in sediment samples ingested and egested by the polychaete, reflected similar differences and trends as total direct counts or plate counts. Nevertheless, application of that viable direct count technique in marine sediments suffered also from considerable systematic errors.
    Type: Article , NonPeerReviewed
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