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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 170 (1999), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An environmental isolate of Salmonella typhi was chromosomally marked with a gfp gene encoding green fluorescence protein (GFP) isolated from Aequorea victoria. The hybrid transposon mini-Tn5 gfp was transconjugated from E. coli to S. typhi, resulting in constitutive GFP production. The survival of S. typhi GFP155 introduced into groundwater and pond water microcosms was examined by GFP-based plate counts, total cell counts, and direct viable counts. A comparison between GFP-based direct viable counts and plate counts was a good method for verifying the viable, but non-culturable (VBNC), state of S. typhi. The entry into a VBNC state of S. typhi was shown in all microcosms. S. typhi survived longer in groundwater than in pond water as both a culturable and a VBNC state.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0972
    Keywords: Bacterial community ; bioremediation ; PCR-SSCP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The extent of shift in soil bacterial community structure during bioremediational treatments was investigated by PCR-single-strand-conformation polymorphism (SSCP) analysis, which was followed by computer-assisted cluster analysis of the community fingerprints. While biostimulation as well as bioaugmentation enhanced the degradation of phenanthrene in soil, both bioremediational treatments caused shifts in the bacterial community structure. Drastic changes were observed in the initial phase of bioaugmentation. Our results demonstrate that computer-assisted fingerprint analysis is readily applicable to the study for the comparative analysis of microbial community structure using molecular profiling techniques.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2017-05-03
    Description: The Norwegian young sea ICE (N-ICE2015) expedition was designed to investigate the atmosphere-snow-ice-ocean interactions in the young and thin sea-ice regime north of Svalbard. Radiosondes were launched twice daily during the expedition from January to June 2015. Here we use these upper-air measurements to study the multiple cyclonic events observed during N-ICE2015 with respect to changes in the vertical thermodynamic structure, moisture content and boundary layer characteristics. We provide statistics of temperature inversion characteristics, static stability and boundary layer extent. During winter, when radiative cooling is most effective, we find the strongest impact of synoptic cyclones. Changes to thermodynamic characteristics of the boundary layer are associated with transitions between the radiatively 'clear' and 'opaque' atmospheric states. In spring, radiative fluxes warm the surface leading to lifted temperature inversions and a statically unstable boundary layer. Further, we compare the N-ICE2015 static stability distributions to corresponding profiles from ERA-Interim reanalysis, from the closest land station in the Arctic North Atlantic sector, Ny-Ålesund, and to soundings from the SHEBA expedition 1997/1998. We find similar stability characteristics for N-ICE2015 and SHEBA throughout the troposphere, despite differences in location, sea-ice thickness and snow cover. For Ny-Ålesund, we observe similar characteristics above 1000 m, while the topography and ice-free fjord surrounding Ny-Ålesund generate great differences below. The long-term radiosonde record 1993-2014) from Ny-Ålesund indicates that during the N-ICE2015 spring period temperatures were close to the climatological mean, while the lowest 3000 m were 1 − 3 ∘ C warmer than the climatology during winter.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
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  • 4
    Publication Date: 2018-05-23
    Description: Genomic and ecological study of two distinctive freshwater bacteriophages infecting a Comamonadaceae bacterium Genomic and ecological study of two distinctive freshwater bacteriophages infecting a 〈i〉Comamonadaceae〈/i〉 bacterium, Published online: 22 May 2018; doi:10.1038/s41598-018-26363-y Genomic and ecological study of two distinctive freshwater bacteriophages infecting a Comamonadaceae bacterium
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
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