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  • Phylogeny  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake (1998)
    Springer
    Extremophiles 2 (1998), S. 359-366 
    Details
    ISSN: 1433-4909
    Keywords: Key wordsDietzia natronolimnaios sp. nov. ; Alkaliphile ; Soda lake ; 16S rDNA ; Phylogeny ; Chemotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two novel alkaliphilic aerobic organotrophic bacteria have been isolated from a moderately saline and alkaline East African soda lake. The new isolates grow at pH values between 6 and 10, with a pH optimum for growth of 9.0, and at a salt concentration between 0% and 10% (w/v). Phylogenetic analysis based on 16S rDNA sequence shows that these isolates are very closely related (99.6% similarity) and are members of the monospecific genus Dietzia (98.8% and 98.7% similarity). DNA/DNA hybridization revealed a relatedness of 83% between the two isolates, but only 8% between them and the type strain Dietzia maris. The G + C content as measured by thermal denaturation is 66.1 mol%. Phenotypic comparisons between D. maris and one isolate showed that they share very similar morphological and chemotaxonomic properties, but differ significantly in carbon source utilization profiles and halotolerance in alkaline medium. We propose a second species of this genus which we name Dietzia natronolimnaios (type strain 15LN1 = CBS 107.95).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050079
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Novel archaeal phylotypes from an East African alkaline saltern (1999)
    Springer
    Extremophiles 3 (1999), S. 139-145 
    Details
    ISSN: 1433-4909
    Keywords: Key words Halophile ; Haloalkaliphile ; Phylogeny ; Phylotype ; 16S rDNA ; Alkaline saltern ; Archaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA has been extracted on site from the brines of the final crystallizing pond of an alkaline saltern at Lake Magadi, Kenya. Amplification of 16S rRNA genes followed by cloning, sequencing, and phylogenetic analysis has revealed the presence of two distinct new archaeal lineages. The majority of cloned sequences showed greater than 95% identity to each other, but only 88%–90% similarity to any cultivated haloalkaliphilic Archaea, and form a distinct cluster within the known Haloarchaea. Two cloned genes showed close similarity to each other but only 76% similarity to any known archaeal sequence, and therefore represent a distinct phylotype only distantly related to the euryarchaeotal branch of the Archaea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050109
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    Paper (German National Licenses)
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