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  • 1
    Electronic Resource
    Electronic Resource
    Pyruvate metabolism of the hyperthermophilic archaebacterium Pyrococcus furiosus (1991)
    Springer
    Archives of microbiology 155 (1991), S. 366-377 
    Details
    ISSN: 1432-072X
    Keywords: Pyrococcus furiosus ; Hyperthermophilic archabacteria ; Pyruvate fermentation ; Growth yields ; Hydrogen inhibition ; Sulfur stimulation ; Pyruvate:ferredoxin oxidoreductase ; Acetyl-CoA synthetase (ADP forming) ; Adenylate kinase ; ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hyperthermophilic anaerobe Pyrococcus furiosus was found to grow on pyruvate as energy and carbon source. Growth was dependent on yeast extract (0.1%). The organism grew with doublings times of about 1 h up to cell densities of 1–2×108 cells/ml. During growth 0.6–0.8 mol acetate and 1.2–1.5 mol CO2 and 0.8 mol H2 were formed per mol of pyruvate consumed. The molar growth yield was 10–11 g cells(dry weight)/mol pyruvate. Cell suspensions catalyzed the conversion of 1 mol of pyruvate to 0.6–0.8 mol acetate, 1.2–1.5 mol CO2, 1.2 mol H2 and 0.03 mol acetoin. After fermentation of [3-14C]pyruvate the specific radioactivities of pyruvate, CO2 and acetate were equal to 1:0.01:1. Cellfree extracts contained the following enzymatic activities: pyruvate: ferredoxin (methyl viologen) oxidoreductase (0.2 U mg-1, T=60°C, with Clostridium pasteurianum ferredoxin as electron acceptor; 1.4 U mg-1 at 90°C, with methyl viologen as electron acceptor); acetyl-CoA synthetase (ADP forming) [acetyl-CoA+ADP+Pi⇆acetate+ATP+CoA] (0.34 U mg-1, T=90°C), and hydrogen: methyl viologen oxidoreductase (1.75 U mg-1). Phosphate acetyl-transferase activity, acetate kinase activity, and carbon monoxide:methyl viologen oxidoreductase activity could not be detected. These findings indicate that the archaebacterium P. furiosus ferments pyruvate to acetate, CO2 and H2 involving only three enzymes, a pyruvate:ferredoxin oxidoreductase, a hydrogenase and an acetyl-CoA synthetase (ADP forming).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00243457
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  • 2
    Electronic Resource
    Electronic Resource
    Growth parameters (K s, μmax, Y s) of Methanobacterium thermoautotrophicum (1980)
    Springer
    Archives of microbiology 127 (1980), S. 59-65 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Growth rates ; Growth yields ; Nickel ; Maintenance coefficient ; Interspecies hydrogen transfer ; Saturation constants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methanobacterium thermoautotrophicum was grown on a mineral salts medium in a fermenter gassed with H2 and CO2, which were the sole carbon and energy sources. Under the conditions used the bacterium grew exponentially. The dependence of the growth rate (μ) on the concentration of H2 and CO2 in the incoming gas and the dependence of the growth yield ( $$Y_{CH_4 }$$ ) on the growth rate were determined at pH 7 (the pH optimum) and 65° C (the temperature optimum). The curves relating growth rate to the H2 and CO2 concentration were hyperbolic. From reciprocal plots apparent K s values for H2 and CO2 and μmax were obtained: app. $$K_{{\text{H}}_{\text{2}} }$$ = 20%; app. $$K_{{\text{CO}}_{\text{2}} }$$ = 11%; μ = 0.69 h-1; t δ (max)=1 h. $$Y_{CH_4 }$$ was 1.6 g mol-1 and almost independent of the growth rate, when the rate of methane formation was not limited by the supply of either H2 or CO2. The yield increased to near 3 g mol-1 when H2 or CO2 were limiting. These findings indicate that methane formation and growth are less tightly coupled at high concentrations of H2 or CO2 in the medium than at low concentrations. The physiological significance of these findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414356
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    Paper (German National Licenses)
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