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  • Atlantic Ocean; Comment; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; Flow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R); flow cytometry; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Prokaryotes; prokaryotic abundance; Replicate; Species; Species, unique identification (Semantic URI); Species, unique identification (URI); Type of study  (1)
  • Brain biopsy  (1)
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  • 1
    ISSN: 1432-1459
    Keywords: Key words AIDS ; Brain biopsy ; Cerebral spinal fluid ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Twenty patients with AIDS who had intracranial lesions underwent both brain biopsy and cerebro-spinal fluid (CSF) examination to compare histological diagnosis with the polymerase chain reaction (CSF-PCR) for the identification of infectious agents. CSF-PCR was performed for herpes simplex virus, varicella zoster virus, cytomegalovirus (CMV), JC virus (JCV), Epstein-Barr virus (EBV), Toxoplasma gondii and Mycobacterium tuberculosis. A definitive diagnosis was obtained by brain biopsy in 14 patients (2 with astrocytoma, 12 with brain infection). CSF-PCR was positive for EBV DNA in 3 of 3 cases of primary cerebral lymphoma, positive for JCV DNA in 6 of 7 biopsy-proven (and one autopsy-proven) cases of progressive multifocal leukoencephalopathy (PML). CSF-PCR was positive for CMV DNA in one biopsy-proven and one autopsy-proven case of CMV encephalitis (the former also had PML) and positive for M. tuberculosis DNA in one case of tuberculous encephalitis. None of the five toxoplasmic encephalitis cases (one definite, four presumptive) were T. gondii DNA positive. There was close correlation between histology and CSF-PCR for CMV encephalitis, PML and PCL. Antitoxoplasma therapy affected the sensitivity of both histological and CSF-PCR methods.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2024-03-19
    Description: A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Prokaryotes were quantified by flow citometry (CytoFLEXflex S, Beckman Coulter) as previously described by Gasol et al. (1999) with our own adjustment of the scatter settings to more accurately resolve these cells with the new CytoFLEX flow cytometry technology. Measurements were performed every month from different parts of the aquaria setup: the input seawater, the reservoir tank (after the filtration of seawater to 5 µm and before the UV lamp) and every experimental aquaria.
    Keywords: Atlantic Ocean; Comment; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; Flow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R); flow cytometry; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Prokaryotes; prokaryotic abundance; Replicate; Species; Species, unique identification (Semantic URI); Species, unique identification (URI); Type of study
    Type: Dataset
    Format: text/tab-separated-values, 1260 data points
    Location Call Number Limitation Availability
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