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  • 1
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    Spexin in the European sea bass, Dicentrarchus labrax : Characterization, brain distribution, and interaction with Gnrh and Gnih neurons
    Wiley ; 2023
    In:  Journal of Comparative Neurology Vol. 531, No. 2 ( 2023-02), p. 314-335
    Details
    In: Journal of Comparative Neurology, Wiley, Vol. 531, No. 2 ( 2023-02), p. 314-335
    Abstract: Spexin (Spx) is a recently characterized neuropeptide implicated in multiple physiological processes in vertebrates, including reproduction, food intake, and regulation of anxiety and stress. Two orthologs (Spx1 and Spx2) are present in some nonmammalian vertebrates, including teleosts. However, information on the distribution of Spx in the brain and its interactions with other neuroendocrine systems in fish is still scarce. In this work, we cloned and sequenced the sea bass ( Dicentrarchus labrax ) Spx1, which included a 27 aa signal peptide and a mature peptide of 14 aa that is C‐terminal amidated. spx1 transcripts were higher in the diencephalon/caudal preoptic area/hypothalamus and medulla but were also detected in the olfactory bulbs, telencephalon/rostral preoptic area, optic tectum/tegmentum, cerebellum/pons, and pituitary. The immunohistochemical study revealed Spx1‐immunoreactive (ir) cells in different nuclei of the preoptic area, habenula, prethalamus, mesencephalic tegmentum and in the proximal pars distalis (PPD) and pars intermedia of the pituitary. Spx1‐ir fibers were widely distributed throughout the brain being particularly abundant in the midbrain and hindbrain, in close contact with tegmental gonadotropin‐releasing hormone 2 (Gnrh2) cells and isthmic gonadotropin‐inhibitory hormone (Gnih) cells of the secondary gustatory nucleus. Moreover, Gnih fibers were observed innervating Spx1‐ir cells lying in several subdivisions of the magnocellular preoptic nucleus and in the lateral nucleus of the valvula, whereas ventrolateral prethalamic Spx1‐ir cells received immunopositive Gnrh2 fibers. In the pituitary, Gnrh1‐ir fibers were observed closely associated with Spx1‐ir cells of the PPD. These results suggest that Spx1 could be involved in both reproductive and nonreproductive (i.e., food intake, behavior) functions in sea bass.
    Type of Medium: Online Resource
    ISSN: 0021-9967 , 1096-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/cne.v531.2
    DOI: 10.1002/cne.25428
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1474879-4
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  • 2
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    LPXRFa peptide system in the European sea bass: A molecular and immunohistochemical approach
    Wiley ; 2016
    In:  Journal of Comparative Neurology Vol. 524, No. 1 ( 2016-01), p. 176-198
    Details
    In: Journal of Comparative Neurology, Wiley, Vol. 524, No. 1 ( 2016-01), p. 176-198
    Abstract: Gonadotropin‐inhibitory hormone (GnIH) is a neuropeptide that suppresses reproduction in birds and mammals by inhibiting GnRH and gonadotropin secretion. GnIH orthologs with a C‐terminal LPXRFamide (LPXRFa) motif have been identified in teleost fish. Although recent work also suggests its role in fish reproduction, studies are scarce and controversial, and have mainly focused on cyprinids. In this work we cloned a full‐length cDNA encoding an LPXRFa precursor in the European sea bass, Dicentrarchus labrax . In contrast to other teleosts, the sea bass LPXRFa precursor contains only two putative RFamide peptides, termed sbLPXRFa1 and sbLPXRFa2. sblpxrfa transcripts were expressed predominantly in the olfactory bulbs/telencephalon, diencephalon, midbrain tegmentum, retina, and gonads. We also developed a specific antiserum against sbLPXRFa2, which revealed sbLPXRFa‐immunoreactive (ir) perikarya in the olfactory bulbs‐terminal nerve, ventral telencephalon, caudal preoptic area, dorsal mesencephalic tegmentum, and rostral rhombencephalon. These sbLPXRFa‐ir cells profusely innervated the preoptic area, hypothalamus, optic tectum, semicircular torus, and caudal midbrain tegmentum, but conspicuous projections also reached the olfactory bulbs, ventral/dorsal telencephalon, habenula, ventral thalamus, pretectum, rostral midbrain tegmentum, posterior tuberculum, reticular formation, and viscerosensory lobe. The retina, pineal, vascular sac, and pituitary were also targets of sbLPXRFa‐ir cells. In the pituitary, this innervation was observed close to follicle‐stimulating hormone (FSH), luteinizing hormone (LH) and growth hormone (GH) cells. Tract‐tracing retrograde labeling suggests that telencephalic and preoptic sbLPXRFa cells might represent the source of pituitary innervation. The immunohistochemical distribution of sbLPXRFa cells and fibers suggest that LPXRFa peptides might be involved in some functions as well as reproduction, such as feeding, growth, and behavior. J. Comp. Neurol. 524:176–198, 2016. © 2015 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0021-9967 , 1096-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/cne.v524.1
    DOI: 10.1002/cne.23833
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1474879-4
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  • 3
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    Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis : Molecular cloning, brain localization and physiological effects
    Wiley ; 2018
    In:  Journal of Comparative Neurology Vol. 526, No. 2 ( 2018-02), p. 349-370
    Details
    In: Journal of Comparative Neurology, Wiley, Vol. 526, No. 2 ( 2018-02), p. 349-370
    Abstract: Recently, gonadotropin‐inhibitory hormone (GnIH) has emerged as an important regulator of reproduction in birds and mammals. This RFamide neuropeptide has neuromodulatory functions and controls the synthesis and/or release of gonadotropin‐releasing hormone (GnRH) and gonadotropins. Although teleosts represent about half of all living vertebrates, scientific and technological advances on the Gnih system in fish are scarce, contradictory, and inconclusive. Research on the fish Gnih system appears necessary to better clarify its role in the neuroendocrine and environmental control of vertebrate reproduction. In this study, we cloned a full‐length sequence for the Gnih precursor of a flatfish, the Senegalese sole, coding for three putative Gnih peptides (ssGnih). We also generated specific antibodies against these ssGnih peptides, and used them to localize Gnih cells and their projections in the brain and pituitary. The expression of gnih was particularly evident in the diencephalon, but also in the olfactory bulbs/cerebral hemispheres, optic tectum/tegmentum, retina, and pituitary. The three antibodies used provided consistent results and showed that ssGnih‐immunoreactive perikarya were present in the olfactory bulbs, ventral telencephalon, caudal preoptic area, dorsal tegmentum and rostral rhombencephalon, and their fibers innervated the brain and pituitary profusely. Intramuscular injection of ssGnih‐3 provoked a significant reduction in gnrh‐3 and lh expression, whereas ssGnih‐2 treatment did not affect transcript levels of the main reproductive genes. Our results reveal the existence of a functional Gnih system in the sole brain, profusely innervating different brain areas and the pituitary gland, which could represent an important factor in the neuroendocrine control of flatfish reproduction.
    Type of Medium: Online Resource
    ISSN: 0021-9967 , 1096-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/cne.v526.2
    DOI: 10.1002/cne.24339
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1474879-4
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  • 4
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    Straying from the flatfish retinal plan: Cone photoreceptor patterning in the common sole ( Solea solea ) and the Senegalese sole ( Solea senegalensis )
    Wiley ; 2020
    In:  Journal of Comparative Neurology Vol. 528, No. 14 ( 2020-10), p. 2283-2307
    Details
    In: Journal of Comparative Neurology, Wiley, Vol. 528, No. 14 ( 2020-10), p. 2283-2307
    Abstract: The retinas of nonmammalian vertebrates have cone photoreceptor mosaics that are often organized as highly patterned lattice‐like distributions. In fishes, the two main lattice‐like patterns are composed of double cones and single cones that are either assembled as interdigitized squares or as alternating rows. The functional significance of such orderly patterning is unknown. Here, the cone mosaics in two species of Soleidae flatfishes, the common sole and the Senegalese sole, were characterized and compared to those from other fishes to explore variability in cone patterning and how it may relate to visual function. The cone mosaics of the common sole and the Senegalese sole consisted of single, double, and triple cones in formations that differed from the traditional square mosaic pattern reported for other flatfishes in that no evidence of higher order periodicity was present. Furthermore, mean regularity indices for single and double cones were conspicuously lower than those of other fishes with “typical” square and row mosaics, but comparable to those of goldfish, a species with lattice‐like periodicity in its cone mosaic. Opsin transcripts detected by quantitative polymerase chain reaction ( sws1 , sws2 , rh2.3 , rh2.4 , lws , and rh1 ) were uniformly expressed across the retina of the common sole but, in the Senegalese sole, sws2 , rh2.4 , and rh1 were more prevalent in the dorsal retina. Microspectrophotometry revealed five visual pigments in the retina of the common sole [S(472), M(523), M(536), L(559), and rod(511)] corresponding to the repertoire of transcripts quantified except for sws1 . Overall, these results indicate a loss of cone mosaic patterning in species that are primarily nocturnal or dwell in low light environments as is the case for the common sole and the Senegalese sole. The corollary is that lattice‐like patterning of the cone mosaic may improve visual acuity. Ecological and physiological correlates derived from observations across multiple fish taxa that live in low light environments and do not possess lattice‐like cone mosaics are congruent with this claim.
    Type of Medium: Online Resource
    ISSN: 0021-9967 , 1096-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/cne.v528.14
    DOI: 10.1002/cne.24893
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1474879-4
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  • 5
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    A half century of fish gonadotropin‐releasing hormones: Breaking paradigms
    Wiley ; 2022
    In:  Journal of Neuroendocrinology Vol. 34, No. 5 ( 2022-05)
    Details
    In: Journal of Neuroendocrinology, Wiley, Vol. 34, No. 5 ( 2022-05)
    Abstract: The field of fish gonadotropin‐releasing hormones (GnRHs) is also celebrating its 50th anniversary this year. This review provides a chronological history of fish GnRH biology over the past five decades. It demonstrates how discoveries in fish regarding GnRH and GnRH receptor multiplicity, dynamic interactions between GnRH neurons, and additional neuroendocrine factors acting alongside GnRH, amongst others, have driven a paradigm shift in our understanding of GnRH systems and functions in vertebrates, including mammals. The role of technological innovations in enabling scientific discoveries is portrayed, as well as how fundamental research in fish GnRH led to translational outcomes in aquaculture. The interchange between fish and mammalian GnRH research is discussed, as is the value and utility of using fish models for advancing GnRH biology. Current challenges and future perspectives are presented, with the hope of expanding the dialogue and collaborations within the neuroendocrinology scientific community at large, capitalizing on diversifying model animals and the use of comparative strategies.
    Type of Medium: Online Resource
    ISSN: 0953-8194 , 1365-2826
    URL: Issue
    URL: Article
    DOI: 10.1111/jne.v34.5
    DOI: 10.1111/jne.13069
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2007386-0
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  • 6
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    Melatonin receptors in the brain of the European sea bass: An in situ hybridization and autoradiographic study
    Wiley ; 2010
    In:  The Journal of Comparative Neurology Vol. 518, No. 17 ( 2010-05-20), p. 3495-3511
    Details
    In: The Journal of Comparative Neurology, Wiley, Vol. 518, No. 17 ( 2010-05-20), p. 3495-3511
    Type of Medium: Online Resource
    ISSN: 0021-9967 , 1096-9861
    URL: Article
    DOI: 10.1002/cne.22408
    Language: English
    Publisher: Wiley
    Publication Date: 2010
    detail.hit.zdb_id: 1474879-4
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  • 7
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    Identification of two arylalkylamine N-acetyltranferase 1 genes with different developmental expression profiles in the flatfish Solea senegalensis : Duality of arylalkylamine N-acetyltransferase 1 in sole
    Wiley ; 2011
    In:  Journal of Pineal Research Vol. 51, No. 4 ( 2011-11), p. 434-444
    Details
    In: Journal of Pineal Research, Wiley, Vol. 51, No. 4 ( 2011-11), p. 434-444
    Type of Medium: Online Resource
    ISSN: 0742-3098
    URL: Issue
    URL: Article
    DOI: 10.1111/jpi.2011.51.issue-4
    DOI: 10.1111/j.1600-079X.2011.00907.x
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2027992-9
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