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  • 1
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    Regional variability in eukaryotic protist communities in the Amundsen Sea (2013)
    CAMBRIDGE UNIV PRESS
    In:  EPIC3Antarctic Science, CAMBRIDGE UNIV PRESS, 25(6), pp. 741-751, ISSN: 0954-1020
    Details
    Publication Date: 2019-07-16
    Description: We determined the composition and structure of late summer eukaryotic protist assemblages along a west–east transect in the Amundsen Sea. We used state-of-the-art molecular approaches, such as automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing, combined with pigment measurements via high performance liquid chromatography (HPLC) to study the protist assemblage. We found characteristic offshore and inshore communities. In general, total chlorophyll a and microeukaryotic contribution were higher in inshore samples. Diatoms were the dominant group across the entire area, of which Eucampia sp. and Pseudo-nitzschia sp. were dominant inshore and Chaetoceros sp. was dominant offshore. At the most eastern station, the assemblage was dominated by Phaeocystis sp. Under the ice, ciliates showed their highest and haptophytes their lowest abundance. This study delivers a taxon detailed overview of the eukaryotic protist composition in the Amundsen Sea during the summer 2010.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 2
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    Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective (2014)
    CAMBRIDGE UNIV PRESS
    In:  EPIC3Antarctic Science, CAMBRIDGE UNIV PRESS, 26(5), pp. 545-553, ISSN: 0954-1020
    Details
    Publication Date: 2019-07-17
    Description: The diet of Antarctic salps was elucidated by investigating their gut content using “Automated Ribosomal Intergenic Spacer Analysis” and 454-pyrosequencing. Salp samples were collected during the Lazarev Krill Study in the Western Weddell Sea (summer 2005/06 and 2007/08, fall 2004, and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai, both occur in the Southern Ocean, can overlap geographically and seasonally. Here we provide evidence that despite the non-selective feeding mechanism, the two co-occuring salp species might have different niches within one given habitat. ARISA-patterns of 93 salp gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate based diet of salps, even though microscopic analyses identified diatoms as the dominant component of salp gut contents.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 3
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    Monitoring microplastics in the atmosphere and cryosphere in the circumpolar North: A case for multi-compartment monitoring (2022)
    CAMBRIDGE UNIV PRESS
    In:  EPIC3Arctic Science, CAMBRIDGE UNIV PRESS, ISSN: 0954-1020
    Details
    Publication Date: 2022-07-07
    Description: The atmosphere and cryosphere have recently garnered considerable attention due to their role in transporting microplastics to and within the Arctic, and between freshwater, marine, and terrestrial environments. While investigating either in isolation provides valuable insight on the fate of microplastics in the Arctic, monitoring both provides a more holistic view. Nonetheless, despite the recent scientific interest, fundamental knowledge on microplastic abundance, and consistent monitoring efforts, are lacking for these compartments. Here, we build upon the work of the Arctic Monitoring and Assessment Programme’s Monitoring Guidelines for Litter and Microplastic to provide a roadmap for multi-compartment monitoring of the atmosphere and cryosphere to support our understanding of the sources, pathways, and sinks of plastic pollution across the Arctic. Overall, we recommend the use of existing standard techniques for ice and atmospheric sampling and to build upon existing monitoring efforts in the Arctic to obtain a more comprehensive pan-Arctic view of microplastic pollution in these two compartments.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , NonPeerReviewed
    Format: application/pdf
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