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  • 1990-1994  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Potential impact of a toxic dinoflagellate (Alexandrium excavatum) bloom on survival of fish and crustacean larvae (1991)
    Springer
    Marine biology 108 (1991), S. 293-301 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the impact of neurotoxins produced by the dinoflagellateAlexandrium excavatum on survival of Atlantic mackerel (Scomber scombrus) and American lobster (Homarus americanus) larvae, respectively reared from eggs and from female lobster, collected in 1988 from the southern Gulf of St. Lawrence, Canada. Sensitivity to the toxins was first verified by exposing larvae of both species to various concentrations of toxicA. excavatum (treatment) and non-toxicA. tamarense (control). Daily mortality rates ranged from 65 to 96% among mackerel larvae directly fed upon toxic cells and reached 36% in postlarvae exposed to toxic microzooplankton. Lobster larvae were apparently immune to the toxins, which they concentrated up to five times relative to vector toxicities. Bioassays conducted on mackerel larvae by exposure to natural plankton samples collected in situ during a bloom of toxicA. excavatum confirmed that exposure to the toxins could also have lethal effects in natural ecosystems. We conclude that the current proliferation of toxic dinoflagellates threatens early survival of finfish larvae and their recruitment to adult populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01344344
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ion-spray mass spectrometry of marine toxins II. Analysis of diarrhetic shellfish toxins in plankton by liquid chromatography/mass spectrometryNRCC No. 31309. (1990)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 4 (1990), S. 206-213 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A combined liquid chromatography/mass spectrometry (LC/MS) method using ion-spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin-1, the principal toxins implicated in cases of diarrhetic shellfish poisoning. The method is used to confirm the presence of okadaic acid in a culture of the dinoflagellate species Prorocentrum concavum and in natural populations of dinoflagellates from eastern Canadian waters. Several analogues of the toxin have also been detected. Quantitative results are comparable to those obtained by high performance liquid chromatography with fluorescence detection. With slight modifications, the LC/MS method is also used to analyse the fluorescent anthrylmethyl ester of okadaic acid. Preliminary tandem mass spectrometric results, obtained using fast-atom bombardment ionization, are presented for okadaic acid.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290040608
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    Paper (German National Licenses)
    Fulltext
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