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  • 1
    Electronic Resource
    Electronic Resource
    Specific glycosaminoglycans support the inhibition of thrombin by plasminogen activator inhibitor 1 (1993)
    s.l. : American Chemical Society
    Biochemistry 32 (1993), S. 1675-1680 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00057a035
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  • 2
    Electronic Resource
    Electronic Resource
    Morphological Aspects of Synaptic Plasticity in Aplysia An Anatomical Substrate for Long-Term Memory (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 627 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb25924.x
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  • 3
    Electronic Resource
    Electronic Resource
    Geomorphology and In-Situ Cosmogenic Isotopes (1994)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Earth and Planetary Sciences 22 (1994), S. 273-317 
    Details
    ISSN: 0084-6597
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ea.22.050194.001421
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  • 4
    Electronic Resource
    Electronic Resource
    Storage protein gene expression in zygotic and somatic embryos of interior spruce (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 89 (1993), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Storage protein gene expression was compared between zygotic and somatic embryos of interior spruce (Picea glauca/engelmanii complex). Somatic embryos, grown on medium containing 40 μM or 10 μM abscisic acid (ABA), and zygotic embryos accumulated IIS legumin, 7S vicilin and 2S albumin storage proteins. Zygotic embryos displayed a rapid, transient period of storage protein accumulation, while somatic embryos differentiated on 40 μM ABA displayed a more prolonged, gradual accumulation, with some accumulation still evident after 9 weeks of maturation. Somatic embryos on 10 μM ABA accumulated storage proteins initially, but these were rapidly degraded as the embryos germinated precociously. Legumin, albumin and vicilin transcripts were detectable in torpedo stage zygotic and somatic embryos, and increased during embryo development. Transcript levels in zygotic embryos increased during cotyledon development, but following maximum dry weight accumulation and moisture loss, transcripts declined rapidly to low levels. In contrast, somatic embryos on 40 μM ABA had high transcript levels for a prolonged period. These levels were still present after 9 weeks of maturation. A decline in storage protein transcripts similar to zygotic embryos was apparent following a mild drying treatment. These results suggest that a decline in storage protein transcripts is stimulated by embryo drying during the later stages of conifer embryogenesis. Low levels of storage protein transcripts also appeared in somatic embryos on 10 μM ABA, but declined during precocious germination. Osmotic stress induced storage protein and storage protein transcript accumulation. This could be partially inhibited by inclusion of the ABA biosynthetic inhibitor, fluridone. However, endogenous ABA levels did not differ significantly between embryos cultured in the presence or absence of fluridone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1993.tb05278.x
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  • 5
    Electronic Resource
    Electronic Resource
    Triage: In austere environments and echeloned medical systems (1992)
    Springer
    World journal of surgery 16 (1992), S. 904-909 
    Details
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Bien que le terme “triage” ait été à l'origine destiné à définir et à s'appliquer aux procédés de sélection des blessés en cas de catastrophes collectives, ce terme s'applique aujourd'hui à pratiquement tous les aspects de la médecine. Cet essai a comme but de définir les grandes lignes du triage en milieu militaire ou en cas de grandes catastrophes, de souligner l'importance de l'hierarchie dans ce système ainsi que d'insister sur les facteurs non médicaux qui peuvent influencer les décisions et les priorités du triage. En raison de l'absence d'expérience analoque en pratique civile, il est essentiel que tout le personnel médical pouvant être intéressé par un système de soins échelonné, acquière et maintienne une connaisance en la matière par une participation active, fréquente des exercises d'entrainement. Les points essentiels de ceux-ci sont décrits ici.
    Abstract: Resumen Aunque el triage, como proceso de selección médica, fue originalmente desarrollado y aplicado en sistemas militares escalafonados de campo que atendían heridos en masa, el término ha sido introducido en la mayoría de los aspectos de la práctica médica civil. Este artículo intenta reenfocar el triage en los escenarios militares y de desastre, haciendo énfasis en la relación entre la organización de los sistemas escalafonados y el proceso de triage, y los diversos factores no médicos que pueden influenciar las decisiones y prioridades del triage. Puesto que no existe una experiencia civil similar, es esencial que la totalidad del personal de salud que pueda verse involucrado en un sistema escalafonado de atención médica adquiera y mantenga experiencia mediante su frecuente participación en buenos programas de capacitación, cuyas características se describen.
    Notes: Abstract Although triage as a medical sorting process was originally developed and applied to echeloned military field medical systems dealing with mass casualties, the term has now permeated most aspects of medical practice. This essay attempts to refocus on triage in military and disaster settings, emphasizing the relationship of the echeloned system organization to the triage process, and the various non-medical factors which may influence triage decisions and priorities. Due to the lack of any analogous civilian experience, it is essential that all health personnel who may be involved in an echeloned care system gain and maintain proficiency by frequent involvement in appropriate training exercises, the characteristics of which are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02066990
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  • 6
    Electronic Resource
    Electronic Resource
    Two-isotope characterization of N20 in the Pacific Ocean and constraints on its origin in deep water (1990)
    [s.l.] : Nature Publishing Group
    Nature 347 (1990), S. 58-61 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Our profiles were made at three of the Pacific GEOSECS stations12: station 314 in the South Pacific gyre, station 204 in the North Pacific gyre and station 347, the Northeast Pacific 'GEOSECS F station13. Seawater samples of total volume -2,000 litres were collected at each depth and stripped of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/347058a0
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  • 7
    Electronic Resource
    Electronic Resource
    He, Sr and Nd isotopic variations in lavas from the juan fernandez archipelago, SE pacific (1993)
    Springer
    Contributions to mineralogy and petrology 115 (1993), S. 75-87 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Helium, Sr, and Nd isotopic ratios and major and trace element compositions have been measured on a suite of lavas from the intra-plate volcanos of the Juan Fernandez Archipelago, Chile. Lavas from the islands of Mas Afuera and Mas a Tierra and from Monte Alpha and Friday seamounts have Sr and Nd isotopic ratios lying on the low143Nd/144Nd side of the mantle array (87Sr/86Sr:0.7034–0.7037;143Nd/144Nd:0.51281–0.51289). The homogeneity of these tracers suggests the involvement of a restricted range of mantle source compositions throughout Juan Fernandez volcanism. In marked contrast is the large range in3He/4He, from 7.8 to 18.0 RA. A bimodal3He/4He distribution on Mas a Tierra is associated with two distinct volcanic lineages;3He/4He ratios of 14.5–18.0 RA (n=15) occur in alkalic and tholeiitic shield basalts, whereas post-shield basanites range from 11.2 to 13.6 RA (n=12). Elemental and isotopic systematics demonstrate a transition from an enriched (Loihi-like) plume source in the shield lavas to a more MORB-like source in the post-shield volcanics. The transition between these sources is much more pronounced in3He/4He than in the other isotopic tracers. The predominantly tholeiitic basalts of Mas Afuera have exceptionally uniform isotopic and elemental characteristics; Sr and Nd ratios are similar to those of Mas a Tierra, but3He/4He ratios are lower and more uniform at 8.3±0.5 RA (n=17). The dramatic distinction between Mas Afuera and Mas a Tierra helium is surprising given the great similarity between the two islands in other geochemical characteristics. Both the Mas Afuera and Mas a Tierra results demonstrate that helium records systematic processes not readily apparent from other isotopic or elemental indicators. Neither magma chamber degassing nor local metasomatic events are likely to be responsible. We suggest that the observed variations may be attributed to mixing of plume and asthenospheric sources in which the plume component is characterized by a heterogeneous distribution of volatiles or has suffered extraction of small degree partial melts prior to mixing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00712980
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  • 8
    Electronic Resource
    Electronic Resource
    Ca2+-activated K+ channels in airway smooth muscle are inhibited by cytoplasmic adenosine triphosphate (1991)
    Springer
    Pflügers Archiv 417 (1991), S. 517-522 
    Details
    ISSN: 1432-2013
    Keywords: Airway smooth muscle ; Patch-clamp ; Ca2+-activated K+ channels ; ATP sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Large-conductance Ca2+-activated K+ channels were studied in membranes of cultured rabbit airway smooth muscle cells, using the patch-clamp technique. In cell-attached recordings, channel openings were rare and occurred only at very positive potentials. Bradykinin (10 μM), an agonist which releases Ca2+ from the sarcoplasmic reticulum, transiently increased channel activity. The metabolic blocker 2,4-dinitrophenol (20 μM), which lowers cellular adenosine triphosphate (ATP) levels, induced a sustained increase of channel activity in cell-attached patches. In excised patches, these channels had a slope conductance of 155 pS at 0 mV, were activated by depolarization and by increasing the Ca2+ concentration at the cytoplasmic side above 10−7 mol/l. ATP, applied to the cytoplasmic side of the patches, dose-dependently decreased the channel's open-state probability. An inhibition constant (K i) of 0.2 mmol/l was found for the ATP-induced inhibition. ATP reduced the Ca2+ sensitivity of the channel, shifting the Ca2+ activation curve to the right and additionally reducing its steepness. Our results demonstrate that cytoplasmic ATP inhibits a large-conductance Ca2+-activated K+ channel in airway smooth muscle. This ATP modulation of Ca2+-activated K+ channels might serve as an important mechanism linking energy status and the contractile state of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00370948
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  • 9
    Electronic Resource
    Electronic Resource
    Linkage analysis of the genetic determinants of high density lipoprotein concentrations and composition: evidence for involvement of the apolipoprotein A-II and cholesteryl ester transfer protein loci (1994)
    Springer
    Human genetics 〈Berlin〉 93 (1994), S. 639-648 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have tested for evidence of linkage between the genetic loci determining concentrations and composition of plasma high density lipoproteins (HDL) with the genes for the major apolipoproteins and enzymes participating in lipoprotein metabolism. These genes include those encoding various apolipoproteins (apo), including apoA-I, apoA-II, apoA-IV, apoB, apoC-I, apoC-II, apoC-III, apoE, and apo(a), cholesteryl ester transfer protein (CETP), HDL-binding protein, lipoprotein lipase, and the low density lipoprotein (LDL) receptor. Polymorphisms of these genes, and nearby highly polymorphic simple sequence repeat markers, were examined by quantitative sib-pair linkage analysis in 30 coronary artery disease families consisting of a total of 366 individuals. Evidence for linkage was observed between a marker locus D16S313 linked to the CETP locus and a locus determining plasma HDL-cholesterol concentration (P = 0.002), and the genetic locus for apoA-II and a locus determining the levels of the major apolipoproteins of HDL, apoA-I and apoA-II (P = 0.009 and 0.02, respectively). HDL level was also influenced by the variation at the apo(a) locus on chromosome 6 (P = 0.02). Thus, these data indicate the simultaneous involvement of at least two different genetic loci in the determination of the levels of HDL and its associated lipoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201563
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  • 10
    Electronic Resource
    Electronic Resource
    Treatment of severe male-factor infertility with high concentrations of motile sperm by microinsemination in embryo cryopreservation straws (1991)
    Springer
    Journal of assisted reproduction and genetics 8 (1991), S. 101-110 
    Details
    ISSN: 1573-7330
    Keywords: microinsemination ; male-factor infertility ; oligo/asthenospermia ; in vitro fertilization (IVF) ; pronuclear-stage embryo transfer (PROST) ; gamete micromanipulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A microinsemination technique was evaluated for treating our program's most severe cases of male-factor infertility. Oocytes were inseminated with high concentrations of motile sperm (1 to 9×106/ml) in 10 to 150 μl within embryo cryopreservation straws. Fertilization was obtained in 20 of 29 (69%) couples treated by this technique. In the 15 patients in which only embryos generated from the straw technique were transferred, 7 clinical pregnancies resulted (46.7% per transfer). The implantation rate for couples receiving embryos from the straw technique only (12/58; 20.7%) compared favorably to that observed for other cases treated during this same time period with regular insemination techniques (111/766; 14.5%). Clinical pregnancy rates per transfer for IVF-ET, TET, and PROST were 33.0% (1/3), 0% (0/2), and 60.0% (6/10), respectively. The percentage of polyploidic embryos was significantly lower (P〈0.0001) for male-factor patients treated by the straw technique with high sperm concentrations that for non-male-factor patients treated during this same time period with standard sperm concentrations. Normal births have resulted from straw inseminations with 3.4×106 and ongoing pregnancies with 5.0×106 motile sperm/ml. The results of this study suggest that some cases of male-factor infertility can be successfully treated by insemination with high concentrations of motile sperm in embryo cryopreservation straws. A technique of centrifuging sperm in straws was also developed to concentrate the entire fraction of washed sperm into 10 μl. Further development of this technique may allow treatment of more severe cases of oligo/asthenospermia by microinsemination with high concentrations of motile sperm than is presently possible with standard washing techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01138663
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