In:
Tropical Journal of Pharmaceutical Research, African Journals Online (AJOL), Vol. 21, No. 3 ( 2022-05-29), p. 555-561
Abstract:
Purpose: To study the impact of Bushen Qudu Decoction on renal function, renal tissue morphology and TGF-β1/Smads signal transduction pathway in adenine-induced chronic renal failure rats.Methods: 76 rats were assigned to normal group, model group, uremic clearance group, benazepil group, Bushen Qudu decoction gunshan A group and Bushen Qudu decoction Guiza group. Renal failure was induced in rats using intragastric administration of adenine for 30 days. The expressions of TGF-β1, Smad2, Smad3 and Smad6 were determined with immunohistochemistry. Real-time quantitative PCR was employed to assay the mRNA expression of TGF-β1 in each group, while protein expressions of Smad2, Smad3 and Smad6 were determined with western blot. The scores of glomerular and tubulointerstitial lesions in each group were obtained by histopathological examination.Results: Bushen Qudu decoction significantly reduced BUN and creatinine in rats with chronic renal failure; furthermore, it also lowered the protein expressions of TGF-β1 and SMAD2/3, but Smad6 protein, relative to control (p 〈 0.05). The TGF-β1mRNA expression was down-regulated (p 〈 0.05), relative to control group.Conclusion: Bushen Qudu decoction reduces renal interstitial fibrosis in rats and delays the progression of chronic renal failure by regulating TGF-β1/Smads signaling pathway. These findings provide some insight that should facilitate the development of new drugs that would delay the onset of chronic renal failure.
Type of Medium:
Online Resource
ISSN:
1596-9827
,
1596-5996
DOI:
10.4314/tjpr.v21i3.15
Language:
Unknown
Publisher:
African Journals Online (AJOL)
Publication Date:
2022
detail.hit.zdb_id:
2125881-8
SSG:
15,3
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