ISSN:
1573-5028
Keywords:
soybean seedlings
;
eEF-1α
;
tef genes
;
cDNA
;
genomic DNA
;
dark/light transcription
;
intron
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A cDNA and a genomic DNA library from soybean (Glycine max L.) were used to identify and sequence two genes coding for the α-subunit of the translation elongation factor eEF-1. Within the coding part, the two genes (tefS1 andtefS2) diverge in 80 wobble positions thus yielding an identical protein composed of 447 amino acids. The soybean protein has about 95% similarity with eEF-1α proteins ofArabidopsis thaliana and tomato. Both genesS1 andS2 contain, within the coding part at a site seemingly unique to higher plants, a single short intron of 86 and 116 nucleotides, respectively. The untranslated leader part of both genes is interrupted by a large intron (partially sequenced). GenesS1 andS2 are transcribed in young leaves. cDNA and gene-specific oligonucleotide probes interact with a unique transcript of close to 1.9 kb. Northern hybridization studies using RNAs from dark- and light-grown seedlings show that light sharply increases the level of stable transcripts (1.9 kb). A peak value is measured after about 3 h of illumination, afterwards the transcript concentration drops to about 10% of the peak value. GenesS1 andS2 follow a similar transcription pattern in developing seedling leaves, which is distinct from that of therbcS genes measured in parallel experiments. According to northern results,S1 transcripts are more abundant in leaves at all measured stages of development thanS2 transcripts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040630
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