Notes: Filamentous microbial mats from three aphotic sulfidic springs in Lower Kane Cave, Wyoming, were assessed with regard to bacterial diversity, community structure, and ecosystem function using a 16S rDNA-based phylogenetic approach combined with elemental content and stable carbon isotope ratio analyses. The most prevalent mat morphotype consisted of white filament bundles, with low C:N ratios (3.5–5.4) and high sulfur content (16.1–51.2%). White filament bundles and two other mat morphotypes had organic carbon isotope values (mean δ13C =−34.7‰, 1σ= 3.6) consistent with chemolithoautotrophic carbon fixation from a dissolved inorganic carbon reservoir (cave water, mean δ13C =−7.4‰ for two springs, n= 8). Bacterial diversity was low overall in the clone libraries, and the most abundant taxonomic group was affiliated with the “Epsilonproteobacteria” (68%), with other bacterial sequences affiliated with Gammaproteobacteria (12.2%), Betaproteobacteria (11.7%), Deltaproteobacteria (0.8%), and the Acidobacterium (5.6%) and Bacteriodetes/Chlorobi (1.7%) divisions. Six distinct epsilonproteobacterial taxonomic groups were identified from the microbial mats. Epsilonproteobacterial and bacterial group abundances and community structure shifted from the spring orifices downstream, corresponding to changes in dissolved sulfide and oxygen concentrations and metabolic requirements of certain bacterial groups. Most of the clone sequences for epsilonproteobacterial groups were retrieved from areas with high sulfide and low oxygen concentrations, whereas Thiothrix spp. and Thiobacillus spp. had higher retrieved clone abundances where conditions of low sulfide and high oxygen concentrations were measured. Genetic and metabolic diversity among the “Epsilonproteobacteria” maximizes overall cave ecosystem function, and these organisms play a significant role in providing chemolithoautotrophic energy to the otherwise nutrient-poor cave habitat. Our results demonstrate that sulfur cycling supports subsurface ecosystems through chemolithoautotrophy and expand the evolutionary and ecological views of “Epsilonproteobacteria” in terrestrial habitats.

Notes: Abstract We measured the C- and O-isotopic composition of carbonate minerals in the Waits River Formation, eastern Vermont, to determine the extent of fluid infiltration during regional metamorphism over an approx. 2000 km2 area in a deep-seated (〉25 km) Barrovian terrane. From a petrologic study of this terrane, Ferry proposed the existence of a large regional metamorphic hydrothermal system with two first-order features: (1) on the scale of the entire terrane fluid flow was focused into the axes of two major antiforms of regional extent; (2) on a smaller scale (about 100 km2) flow was further focused around synmetamorphic granitic plutons that intruded along the axes of the antiforms. We find isotopic evidence for both the regional hydrothermal activity along the antiforms and the more intense fluid flow around synmetamorphic plutons. The evidence for hydrothermal activity around the plutons is large heavy isotope depletions, up to 6–9‰ in δ18Ocarb and δ13Ccarb, in diopside zone rocks adjacent to the plutons. These isotopic shifts are greater than can be explained solely by prograde metamorphic reactions. We find two lines of evidence for the more diffuse regional flow that was focused into axes of the antiforms. First, δ18Ocarb and δ13Ccarb, within individual outcrops become increasingly homogeneous with increasing grade towards antiform axes, indicating that the rocks equilibrated with a permeating fluid. Second, there are depletions in 18O near the margins of the Waits River Formation which can be interpreted as a dispersed, advective infiltration front displaced toward the antiform axes. These fronts were modelled using Eq. 13 of Bickle and Baker and imply time-integrated fluid of 105–106 cm3/cm2, which are consistent with values derived by Ferry from measured progress of prograde devolatilization reactions.