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  • 1
    Electronic Resource
    Electronic Resource
    An expression vector system providing plasmid stability and conditional suicide of plasmid-containing cells (1992)
    Springer
    Applied microbiology and biotechnology 38 (1992), S. 91-93 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A cloning vector system was constructed on the basis of the pBR322 derivative pEG1 by introducing the whole parB locus of plasmid R1 cloned behind the promoter of the alkaline phosphatase gene (phoA) of Escherichia coli. The parB locus in combination with the phoA promoter ensures both (i) plasmid stabilization due to the post-segregational killing of plasmid-free cells during growth and (ii) killing of the cells induced by the potential environmental signal phosphate limitation. This vector, therefore, appears to be a model system for increasing the stability of recombinant plasmids and for decreasing the potential risks in the application of recombinant bacteria in industrial fermentations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169425
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization and organization of phenol degradation genes ofPseudomonas putida strain H (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 240-246 
    Details
    ISSN: 1617-4623
    Keywords: Pseudomonas putida ; Phenol degradation ; Insertion mutagenesis ; Gene organization ; DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genetic organization of the DNA region encoding the phenol degradation pathway ofPseudomonas putida H has been investigated. This strain can utilize phenol or some of its methylated derivatives as its sole source of carbon and energy. The first step in this process is the conversion of phenol into catechol. Catechol is then further metabolized via themeta-cleavage pathway into TCA cycle intermediates. Genes encoding these enzymes are clustered on the plasmid pPGH1. A region of contiguous DNA spanning about 16 kb contains all of the genetic information necessary for inducible phenol degradation. The analysis of mutants generated by insertion of transposons and cassettes indicates that all of the catabolic genes are contained in a single operon. This codes for a multicomponent phenol hydroxylase andmeta-cleavage pathway enzymes. Catabolic genes are subject to positive control by the gene product(s) of a second locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00705655
    Location Call Number Limitation Availability
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    Paper (German National Licenses)
    Fulltext
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