Publication Date:
2024-04-02
Description:
Sediment cores from sites K3, K4, K5, and K7 were recovered by subsampling a box corer (50 × 50 cm), while sediment cores from sites K4 and K6 were collected using an autonomous lander system. Microbes and virus-like particles were extracted from sediments in a 3°C room using a modified version of the washing protocol of Danovaro and Middelboe, 2010 (see Schauberger et al., 2021). After the washing procedure, the extracted microbial cells and virus-like particles were fixed with 25% glutaraldehyde (1% final concentration) and stored at −80°C prior to flow cytometry. These samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I. Sediment extracts were diluted 1 : 10 in 0.02 μm-filtered TE Buffer prior to all measurements. The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).
Keywords:
Abundance; Bacteria; BC; Box corer; Carbon, organic, total; Date/Time of event; Deep sea; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Elevation of event; Event label; flow cytometry; Flow cytometry system, Becton Dickinson, FACSCanto II; Hadal trench; Kermadec Trench; Latitude of event; Longitude of event; Microbial abundance, cells; Microbial abundance, standard deviation; MUC; MultiCorer; organic matter; prokaryote; sediment; Site K3; Site K4; Site K5; Station label; TAN1711; TAN1711_K3; TAN1711_K4-1; TAN1711_K5-1; TAN1711_K6; TAN1711_K7; Tangaroa; Viral abundance; Viral abundance, standard deviation; virus-like particles
Type:
Dataset
Format:
text/tab-separated-values, 914 data points
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