Description: The excel file has two spread-sheets: (i) "Microsatellites-4Areas" including the information of the 11 microsatellites used in the paper for the four different areas we investigated; (ii) "Microsatellites-30Populations" including the information of the 11 microsatellites used in the paper for the 30 different populations we investigated. In the two spread-sheets we include the following columns: "Number of individual" from 1 to 168; "Area" with the names of the areas or populations for every individual; "Sample Code" with the name of the sample used in the paper; "1Ple, 3Ple, 11Ple, 13Ple, 12Ple, 14Ple, 16Ple, 5Ple, 19Ple, 10Ple, and 2Ple" the name of each of the 11 microsatellites gentotyped in our study. In addition to that, we provide a small summary of the "Number of microsatellites", "Number of Individuals", "Number of Areas", "Number of Populations", and "N of individuals per area and population".

Description: Sponges are interesting animal models for regeneration studies, since even from dissociated cells, they are able to regenerate completely. In particular, explants are model systems that can be applied to many sponge species, since small fragments of sponges can regenerate all elements of the adult, including the oscula and the ability to pump water. The morphological aspects of regeneration in sponges are relatively well known, but the molecular machinery is only now starting to be elucidated for some sponge species. Here, we have used an explant system of the demosponge Halichondria panicea to understand the molecular machinery deployed during regeneration of the aquiferous system. We sequenced the transcriptomes of four replicates of the 5–day explant without an osculum (NOE), four replicates of the 17–18–day explant with a single osculum and pumping activity (PE) and also four replicates of field–collected individuals with regular pumping activity (PA), and performed differential gene expression analysis. We also described the morphology of NOE and PE samples using light and electron microscopy. Our results showed a highly disorganised mesohyl and disarranged aquiferous system in NOE that is coupled with upregulated pathways of ciliogenesis, organisation of the ECM, and cell proliferation and survival. Once the osculum is formed, genes involved in “response to stimulus in other organisms” were upregulated. Interestingly, the main molecular machinery of vasculogenesis described in vertebrates was activated during the regeneration of the aquiferous system. Notably, vasculogenesis markers were upregulated when the tissue was disorganised and about to start forming canals (NOE) and angiogenic stimulators and ECM remodelling machineries were differentially expressed once the aquiferous system was in place (PE and PA). Our results are fundamental to better understanding the molecular mechanisms involved in the formation of the aquiferous system in sponges, and its similarities with the early onset of blood-vessel formation in animal evolution.