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  • 1
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA of Chlamydomonas reinhardtii: the DNA sequence of a region showing homology with mammalian URF2 (1984)
    Springer
    Current genetics 9 (1984), S. 75-82 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA of an alga ; Chlamydomonas reinhardtii ; DNA sequence ; URF2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 1.27 kb DNA fragment of the 15 kb DNA of Chlamydomonas reinhardtii has been cloned and sequenced. A 906 bp long open reading frame was found showing homology with the URF2 genes of mammals and insects. This homology is functional evidence for Chlamydomonas reinhardtii 15 kb DNA representing indeed mitochondrial DNA. This is the first report of an URF2 gene in mitochondria of a photosynthetic organism. The absence of a TGA codon within the gene suggests that it is used as stop codon like in higher plants and not as tryptophan like in animal and fungal mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396207
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  • 2
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA of Chlamydomonas reinhardtii: the ND4 gene encoding a subunit of NADH dehydrogenase (1989)
    Springer
    Current genetics 16 (1989), S. 61-64 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA ; NADH dehydrogenase subunit ND4 ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ND4 gene encoding a subunit of respiratory NADH dehydrogenase has been identified on the linear 15.8 kb mitochondrial DNA of Chlamydomonas reinhardtii. The gene maps downstream of ND5. The 1,332 bp nucleotide sequence presented is the first complete reported ND4 sequence from a photoautotrophic organism. The deduced protein of 443 amino acid residues shows 34%, 29% and 27% homology to the protein sequences of Aspergillus amstelodami, Drosophila yakuba and mouse, respectively. ND4 is the fifth and last mitochondrial gene of the NADH dehydrogenase complex on the 15.8 kb mitochondrial genome of C. reinhardtii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411086
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  • 3
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA of Chlamydomonas reinhardtii: the structure of the ends of the linear 15.8-kb genome suggests mechanisms for DNA replication (1993)
    Springer
    Current genetics 24 (1993), S. 241-247 
    Details
    ISSN: 1432-0983
    Keywords: Linear mitochondrial genome ; Terminal inverted repeats ; Internal repeats of terminal sequences ; Long 3′ single-stranded extensions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mitochondrial genome of Chlamydomonas reinhardtii is a linear double-stranded DNA of 15.8 kb. With the exception of the termini its DNA sequence has been published. Here we describe the unique structure of the two termini determined from cloned fragments or, for the very terminal sequences, by the Maxam and Gilbert method after 5′ labeling of uncloned terminal fragments. The 15.8-kb DNA is characterized by terminal inverted repeats of 531 or 532 bp in length including long 3′ extensions. The 3′ single-stranded extensions of the left and right ends are non-complementary, identical in sequence, and comprise 39 to 41 nucleotides. Remarkably, the linear genome possesses in addition an internal 86-bp repeat of the two outermost sequences. The unusual structure of the 15.8-kb DNA termini is compared with those of other linear mitochondrial DNAs. Possible mechanisms of 15.8-kb DNA replication are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351798
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  • 4
    Electronic Resource
    Electronic Resource
    PET1402, a nuclear gene required for proteolytic processing of cytochrome oxidase subunit 2 in yeast (1994)
    Springer
    Molecular genetics and genomics 245 (1994), S. 272-278 
    Details
    ISSN: 1617-4623
    Keywords: Mitochondria ; Protein sorting ; Cytochrome oxidase ; Post-translational proteolysis ; Chromosome V
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nuclear mutation pet ts1402 prevents proteolytic processing of the precursor of cytochrome oxidase subunit 2 (cox2) in Saccharomyces cerevisiae. The structural gene PET1402 was isolated by genetic complementation of the temperature-sensitive mutation. DNA sequence analysis identified a 1206-bp open reading frame, which is located 215 by upstream of the PET122 gene. The DNA sequence of PET1402 predicts a hydrophobic, integral membrane protein with four transmembrane segments and a typical mitochondrial targeting sequence. Weak sequence similarity was found to two bacterial proteins of unknown function. Haploid cells containing a null allelle of PET1402 are respiratory deficient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290106
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  • 5
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA of Chlamydomonas reinhardtii: sequence and arrangement of URF5 and the gene for cytochrome oxidase subunit I (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 213-224 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the present study a new method is described for isolation of mitochondrial DNA from the cell wall-less mutant of Chlamydomonas reinhardtii strain CW15. Using this method the 15 kb DNA was the only DNA species extractable from purified mitochondria and must therefore represent the mitochondrial genome of this species. We present the DNA sequence of a 3.87 kb fragment of the 15 kb mitochondrial DNA. This fragment contains one open reading frame (1623 bp) homologous to the mammalian URF5 and the gene (1518 bp) for subunit I of cytochrome oxidase. The two genes are not interrupted by introns. URF5 is located on the strand opposite to that of the gene for cytochrome oxidase subunit I and of URF2, previously sequenced (Pratje et al. 1984). Therefore, both DNA strands must be transcribed in Chlamydomonas reinhardtii mitochondria. We show the transcripts of all three genes. The deduced amino acid sequences of URF5 and cytochrome oxidase subuit I of Chlamydomonas reinhardtii show 26% and 57% homology to the respective human sequences. A significant bias in the usage of codons is observed for all three genes. The invariant amino acids of cytochrome oxidase subunit I and the gene products of URF2 and URF5 from several organisms have been compared with the respective sequences from Chlamydomonas reinhardtii. Unlike other mitochondrial genomes no deviations from the “universal” genetic code are detectable in Chlamydomonas rein-hardtii mitochondrial DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425662
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  • 6
    Electronic Resource
    Electronic Resource
    Sporulation of mitochondrial respiratory deficient mit - mutants of Saccharomyces cerevisiae (1979)
    Springer
    Molecular genetics and genomics 176 (1979), S. 411-415 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The role of mitochondrial protein synthesis, electron transport, and four specific mitochondrial gene products on sporulation were studied in respiratory deficient mit - mutants. These mutants were isolated in an op1 strain and localized on the mitochondrial genome by petite deletion mapping. All 153 mutations studied could be assigned to the four mitochondrial regions OXI1, OXI2, OXI3 and COB, known to affect cytochrome c oxidase and cytochrome b. The specific loss of one mitochondrially translated polypeptide was found in some mutants of each locus: OXI1—cytochrome c oxidase subunit 2, OXI2 — subunit 3, OXI3 — subunit 1, and COB — cytochrome b. The ability of diploid mit - mutants to sporulate was systematically investigated. About one third of the mutants, representing three loci, were incapable of forming spores. All other cultures produced either respiratory competent mit + tetrads, both mit + and mit - tetrads, or only mit - tetrads. Mutants forming mit - tetrads mapped in all four loci. These results demonstrate that in contrast to petite mutants some mit - mutants have retained the ability to perform meiosis and sporulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333105
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  • 7
    Electronic Resource
    Electronic Resource
    Mapping of the two mitochondrial antimycin a resistance loci in Saccharomyces cerevisiae (1977)
    Springer
    Molecular genetics and genomics 156 (1977), S. 79-85 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Retention or loss of the two new mitochondrial antimycin A resistance loci AI and AII has been analyzed in a large number of stable cytoplasmic petite mutants. Using these deletion mutants it was possible to localize the two antimycin A resistance loci in the OI-OII region of mitochondrial DNA. The genetic loci are mapped in the following order: OII-AI-AII-cobl-OI. The mapping relationship of mutants resistant to antimycin A or funiculosin to various cob mutants is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272255
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  • 8
    Electronic Resource
    Electronic Resource
    Allelism studies of mitochondrial mutants resistant to antimycin A or funiculosin in Saccharomyces cerevisiae (1977)
    Springer
    Molecular genetics and genomics 152 (1977), S. 167-174 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A rapid procedure has been employed to isolate a large number of mitochondrial mutants resistant to antimycin A or funiculosin. A total of 15 antimycin A resistance mutations has been mapped by allelism tests. The mutations belong to two new mitochondrial loci, designated AI and AII. All funiculosin resistance mutations studied up to now map at locus AII. Thus mitochondrial funiculosin resistance might allow the specific selection of mutations in AII. Recombination between the two antimycin A resistance loci AI and AII occurs at frequencies from 8 to 21%. Apparently the two loci are not linked to PAR1, RIB1, RIB3, OLI1, and OLI2. Mutants of the two loci AI and AII have been characterized by measurements of oxygen consumption. Analysis of cytochrome spectra indicates that the mutations affect the cytochrome bc1 complex of the mitochondrial respiratory chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268814
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  • 9
    Electronic Resource
    Electronic Resource
    A GC cluster repeat is a hotspot for mit− macro-deletions in yeast mitochondrial DNA (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 233-240 
    Details
    ISSN: 1617-4623
    Keywords: Yeast mtDNA sequences ; OXI3 gene ; mit deletions ; GC clusters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a random collection of mit− mutations of the yeast strain 777-3A we find that deletions are exceptionally frequent in the OXI3 gene, a large mosaic gene coding for subunit I of cytochrome oxidase. About 10% of all oxi3 − mutants carry the same macro-deletion, del-A, extending from the 5′ non-translated leader of OXI3 to intron 5b of this gene. Determination of the respective wild-type sequences and of the del-A junction sequence revealed that the end-points of the deletion are in two GC clusters with 31 by sequence identity which are located at a distance of 11.3 kb. We speculate that not only the sequence identity of the two GC clusters but also the palindromic structure of these putatively mobile elements of yeast mitochondrial DNA (mtDNA) plays a role in deletion formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273608
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  • 10
    Electronic Resource
    Electronic Resource
    Mitochondrial inner membrane protease 1 of Saccharomyces cerevisiae shows sequence similarity to the Escherichia coli leader peptidase (1991)
    Springer
    Molecular genetics and genomics 228 (1991), S. 167-176 
    Details
    ISSN: 1617-4623
    Keywords: Mitochondria ; Yeast ; Protein targeting ; PET2858 ; Inner membrane protease 1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nuclear yeast mutant pet ts2858 is defective in the removal of pre-sequences from the mitochondrially encoded cytochrome oxidase subunit II (COXII) and the processing intermediate of cytochrome b 2 (Cytb 2), a nuclear gene product. In order to identify the genetic lesion in this mutant we have cloned and characterized a DNA region which complements the pet ts2858 mutation. The DNA sequence revealed three open reading frames, one of which is responsible for the complementation. A 570 by reading frame represents the structural gene PET2858, as demonstrated by in vitro mutagenesis, gene expression from a foreign promoter, and allelism tests. PET2858 encodes a 21.4 kDa protein, which is essential for growth on non-fermentable carbon sources and for the proteolytic processing of COXII and the Cytb 2 intermediate. When the N-terminus of the PET2858 protein is fused to a reporter protein, the resulting hybrid molecule is imported into mitochondria. Interestingly, the N-terminal half of the deduced PET2858 protein exhibits 30.7% amino acid identity to the leader peptidase of Escherichia coli. These results suggest that PET2858 codes for a mitochondrial inner membrane protease (IMP1) or at least a subunit of it. This protease is involved in protein processing and export from the mitochondrial matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282462
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