ISSN:
0006-3592
Keywords:
Acrosiphonia
;
macroalgae
;
tissue culture
;
stirred-tank bioreactor
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A semidifferentiated tissue culture consisting of linear filaments in liquid suspension was established from Acrosiphonia coalita, a cold-water green macroalga known to express pharmacologically active oxylipins deriving from lipoxygenase metabolism of linolenic acid. The tissue was vegatively propagated by blending the filaments down to 1 to 5 mm in length prior to subculture. The filamentous A. coalita tissue suspension was successfully cultivated in an illuminated, 3-L stirred-tank bioreactor at 12°C, 0.46-vvm aeration rate, 250-rpm mixing speed, and incident illumination intensity of 77 μE m-2s-1. The mean specific growth rate over the exponential phase was 0.185 day-1 and a final cell density of 1083 mg dry cell weight (DCW) L-1 was achieved within 15 days of cultivation from an initial cell density of 200 mg DCW L-1. The addition of 3500 ppm CO2 to the aeration gas provided a maximum CO2 transfer rate of six times the maximum CO2 consumption rate and stabilized the pH to 8.0 during the light phase of growth, but did not improve biomass productivity. © 1996 John Wiley & Sons, Inc.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
Permalink
