Note: Cover -- Carbohydrate Bioengineering: Proceedings of an International Conference Elsinore, Denmark, -- Copyright -- Preface -- Table of Contents -- Chapter 1: Glycans of glycoconjugates as modulatory and recognition molecules -- 1. INTRODUCTION -- 2. METHODOLOGY -- 3. MODULATION OF PHYSICOCHEMICAL PROPERTIES -- 4. MODULATION OF BIOLOGICAL ACTIVITY -- 5. ACTIVITIES OF FREE OLIGOSACCHARIDES -- 6. CARBOHYDRATES AS RECOGNITION DETERMINANTS -- 7. OPEN QUESTIONS -- 8. ACKNOWLEDGEMENT -- 9. REFERENCES -- Chapter 2: NMR studies of the structure and dynamics of carbohydrates in aqueous solution -- 1. INTRODUCTION -- 2. HYDROGEN BONDING -- 3. HETERONUCLEAR LONG-RANGE COUPLINGS MEASURED BY A TWO-DIMENSIONAL H.S.M.B.C. EXPERIMENT -- 4. DYNAMICS OF MEMBRANE-BOUND CARBOHYDRATES -- 5. CONCLUSIONS -- 6. ACKNOWLEDGMENTS -- 7. ABBREVIATIONS -- 8. REFERENCES -- Chapter 3: Linkage analysis by mass spectrometry of chemically modified oligo-saccharides from glycosphingolipids and glycoproteins -- 1. INTRODUCTION -- 2. MASS SPECTROMETRY -- 3. CHEMICAL MODIFICATIONS -- 4. GLYCOSPHINGOLIPIDS -- 5. GLYCOPROTEINS -- 6. CONCLUSION -- 7. REFERENCES -- Chapter 4: Development of a novel enzyme based glucose sensor -- 1. INTRODUCTION -- 2. EXPERIMENTAL -- 3. RESULTS AND DISCUSSION -- 4 . REFERENCES -- Chapter 5: Carbohydrate binding at the active site of Escherichia coli maltodextrin phosphorylase -- 1. INTRODUCTION -- 2. SUBSTRATE SPECIFICITY OF MALTODEXTRIN PHOSPHORYLASE -- 3. FUNCTIONAL ROLE OF ACTIVE SITE RESIDUES IN OLIGOSACCHARIDE BINDING -- 4. ACKNOWLEDGEMENT -- 5. REFERENCES -- Chapter 6: The chitinolytic system of Streptomyces olivaceoviridis -- 1. INTRODUCTION -- 2. RESULTS AND DISCUSSION -- 3. ACKNOWLEDGEMENTS -- 4. REFERENCES. , Chapter 7: Properties and production of the β-glycosidase from the thermophilic Archaeon Sulfolobus solfataricus expressed in mesophilic hosts -- 1. INTRODUCTION -- 2. HETEROLOGOUS EXPRESSION -- 3. DOWNSTREAM PROCESSING -- 4. GST-Sβgly FUSIONS: EXPRESSION AND PURIFICATION -- 5. CONCLUSIONS -- 6. ACKNOWLEDGEMENTS -- 5. REFERENCES -- Chapter 8: Contribution of subsites to catalysis and specificity in the extended binding cleft of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases -- 1. INTRODUCTION -- 2. RESULTS -- 3. ACKNOWLEDGEMENTS -- 4. REFERENCES -- Chapter 9: Probing of glycosidase active sites through labeling, mutagenesis and kinetic studies -- 1. INTRODUCTION -- 2. CARBOXYLIC ACIDS PLAYING A ROLE IN CATALYSIS -- 3. MODIFYING THE MECHANISM -- 4. ACKNOWLEDGEMENTS -- 5. REFERENCES -- Chapter 10: Thiooligosaccharides: toys or tools for the studies of glycanases -- 1. INTRODUCTION -- 2. SYNTHESIS OF THIOOLIGOSACCHARIDES -- 3. CONCLUSION -- 4. ACKNOWLEDGEMENTS -- 5. REFERENCES -- Chapter 11: Mutational analysis of catalytic mechanism and specificity in amylolytic enzymes -- 1. INTRODUCTION -- 2. SITE-DIRECTED MUTAGENESIS OF A. NIGER GLUCOAMYLASE -- 3. GENETIC AND CHEMICAL STUDIES OF BARLEY AMYLASE -- 5. CONCLUSION -- 6. FUTURE CHALLENGES -- 7. ACKNOWLEDGEMENTS -- 8. REFERENCES -- Chapter 12: The structure and function relationship of Schizophyllum commune xylanase A -- 1. INTRODUCTION -- 2. ENZYME ISOLATION AND PURIFICATION -- 3. PHYSICO-CHEMICAL PROPERTIES -- 4. KINETICS -- 5. STEREOCHEMISTRY -- 6. DIFFERENCE SPECTROSCOPY -- 7. CHEMICAL MODIFICATION -- 8. SEQUENCE ALIGNMENTS AND STRUCTURE PREDICTIONS -- 9. CONCLUDING REMARKS -- 10. ACKNOWLEDGEMENTS -- 11. REFERENCES -- Chapter 13: Protein engineering of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 -- 1. INTRODUCTION -- 2. METHODS -- 3. RESULTS -- 4. DISCUSSION -- 5. REFERENCES. , Chapter 14: Oxidation stable amylases for detergents -- 1. INTRODUCTION -- 2. RESULTS -- 3. CONCLUSION -- 4. REFERENCES -- Chapter 15: Electrostatic studies of carbohydrate active enzymes -- 1. INTRODUCTION -- 2. BASIC CONCEPTS OF PROTEIN ELECTROSTATICS -- 3. ELECTROSTATIC PROPERTIES OF α-AMYLASES -- 4. APPLICATION TO PHOSPHOGLUCOMUTASE -- 5. CONCLUSIONS -- 6. ACKNOWLEDGEMENTS -- 7. REFERENCES -- Chapter 16: Effects of glycosylation on protein folding, stability and solubility. Studies of chemically modified or engineered plant and fungal peroxidases -- 1. INTRODUCTION -- 2. CHEMICAL DEGLYCOSYLATION OF HORSERADISH PEROXIDASE -- 3. ENGINEERED GLYCO-MUTANTS OF COPRINUS PEROXIDASE -- 4. CONCLUSIONS -- 5. ACKNOWLEDGEMENTS -- 6. REFERENCES -- Chapter 17: Modes of action of two Trichoderma reesei cellobiohydrolases -- 1. INTRODUCTION -- 2. CATALYTIC DOMAIN STRUCTURES OF T. reesei CELLOBIOHYDROLASES -- 3. THE ACTIVE SITE OF CBHII -- 4. THE CELLULOSE-BINDING DOMAIN OF CBHI -- 4. THE INTERDOMAIN LINKER PEPTIDE OF CBHI -- 5. REFERENCES -- Chapter 18: Structural studies on fungal endoglucanases from Humicola insolens -- 1. INTRODUCTION -- 2. ENDOGLUCANASE V -- 3. ENDOGLUCANASE I -- 5. REFERENCES -- Chapter 19: The catalytic domain of endoglucanase A from Clostridium cellulolyticum belonging to family 5: α/β-barrel enzyme -- 1. INTRODUCTION -- 2. THE ENDOGLUCANASE A -- 3. RELATIONSHIP TO OTHER α/β-BARREL GLYCOSYL HYDROLASES -- 4. COMPARISON WITH CELLULASE AND XYLANASE STRUCTURES -- 5. CONCLUSION -- 6. REFERENCES -- Chapter 20: Celluosome domains for novel biotechnological application -- 1. INTRODUCTION -- 2. RESULTS AND DISCUSSION -- 3. REFERENCES -- Chapter 21: Interactions of cellulases from Cellulomonas fimi with cellulose -- 1. INTRODUCTION -- 2. THE CELLULASE SYSTEM OF CELLULOMONAS FIMI -- 3. ANALYSIS OF INTERACTIONS WITH CELLULOSE. , 4. HYDROLYTIC ACTIVITIES OF THE ENZYMES -- 5. ADSORPTION OF THE CBDs TO CELLULOSE -- 6. NATURE OF THE BINDING OF CBDs TO CELLULOSE -- 7. FIBRONECTIN TYPE III (Fn3) REPEATS -- 8. CONCLUSIONS -- 9. ACKNOWLEDGEMENTS -- 10. REFERENCES -- Chapter 22: Transgenic plants as a tool to understand starch biosynthesis -- 1. INTRODUCTION -- 2. RESULTS AND DISCUSSION -- 3. REFERENCES -- Chapter 23: Targeted expression of microbial cellulases in transgenic animals -- 1. INTRODUCTION -- 2. RESULTS -- 3. CONCLUSION -- 4. ACKNOWLEDGEMENTS -- 5. REFERENCES -- Chapter 24: Mechanism and action of glucansucrases -- 1. SOURCES AND STRUCTURES OF THE GLUCANS SYNTHESIZED FROM SUCROSE BY GLUCANSUCRASES -- 2. MECHANISM OF GLUCAN SYNTHESIS -- 3. SYNTHESIS OF BRANCH LINKAGES IN LEUC. MESENTEROIDES B-512F DEXTRAN -- 4. ACCEPTORS AND THE ACCEPTOR-REACTION OF GLUCANSUCRASES -- 5. SUMMARY -- 6. REFERENCES -- Chapter 25: Studies of recombinant amylosucrase -- 1. INTRODUCTION -- 2. MATERIALS AND METHODS -- 3. RESULTS AND DISCUSSION -- 4. CONCLUSION -- 5. ACKNOWLEDGEMENTS -- 6. REFERENCES -- Chapter 26: Application of cloned monocomponent carbohydrases for modification of plant materials -- 1. INTRODUCTION -- 2. MATERIALS AND METHODS -- 3. RESULTS AND DISCUSSION -- 4. CONCLUSIONS -- 5. ACKNOWLEDGEMENTS -- 6. FOOTNOTES -- 7. REFERENCES -- Chapter 27: Fatty acid esters of ethyl glucoside, a unique class of surfactants -- 1. SYNTHESIS -- 2. APPLICATIONS -- 3. PROPERTIES -- 4. ACKNOWLEDGEMENTS -- 5. REFERENCES -- Chapter 28: A wide range of carbohydrate modifications by a single microorganism: leuconostoc mesenteroides -- 1. INTRODUCTION -- 2. DEXTRAN FERMENTATION -- 3. LEUCROSE BIOSYNTHESIS -- 4. MANNITOL FERMENTATION -- 5. ENZYMATIC SYNTHESIS OF GLUCOSE-1-PHOSPHATE -- 6. CONCLUSION -- 7. REFERENCES -- Index.