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  • 1
    In: Geologische Rundschau, Berlin : Springer, 1910, 98(2009), 3, Seite 677-695, 0016-7835
    In: volume:98
    In: year:2009
    In: number:3
    In: pages:677-695
    Type of Medium: Online Resource
    Pages: Ill., graph. Darst
    ISSN: 0016-7835
    Language: English
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  • 2
    In: International journal of earth sciences, Berlin : Springer, 1999, (2007), 1437-3262
    In: year:2007
    In: extent:19
    Type of Medium: Online Resource
    Pages: 19 , Ill., graph. Darst
    ISSN: 1437-3262
    Language: English
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  • 3
    Keywords: Forschungsbericht
    Type of Medium: Online Resource
    Pages: 1 Onlineressource
    Series Statement: Berichte aus dem MARUM und dem Fachbereich Geowissenschaften der Universtiät Bremen no 317
    Language: English
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  • 4
    In: Marine and petroleum geology, Amsterdam [u.a.] : Elsevier Science, 1984, 26(2009), 9, Seite 1812-1823, 0264-8172
    In: volume:26
    In: year:2009
    In: number:9
    In: pages:1812-1823
    Description / Table of Contents: The sediment temperature distribution at mud volcanoes provides insights into their activity and into the occurrence of gas hydrates. If ambient pressure and temperature conditions are close to the limits of the gas hydrate stability field, the sediment temperature distribution not only limits the occurrence of gas hydrates, but is itself influenced by heat production and consumption related to the formation and dissociation of gas hydrates. Located in the Sorokin Trough in the northern Black Sea, the Dvurechenskii mud volcano (DMV) was in the focus of detailed investigations during the M72/2 and M73/3a cruises of the German R/V Meteor and the ROV Quest 4000 m in February and March 2007. A large number of in-situ sediment temperature measurements were conducted from the ROV and with a sensor-equipped gravity corer. Gas hydrates were sampled in pressurized cores using a dynamic autoclave piston corer (DAPC). The thermal structure of the DMV suggests a regime of fluid flow at rates decreasing from the summit towards the edges of the mud volcano, accompanied by intermittent mud expulsion at the summit. Modeled gas hydrate dissociation temperatures reveal that the gas hydrates at the DMV are very close to the stability limits. Changes in heat flow due to variable seepage rates probably do not result in changes in sediment temperature but are compensated by gas hydrate dissociation and formation.
    Type of Medium: Online Resource
    Pages: graph. Darst
    ISSN: 0264-8172
    Language: English
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  • 5
    In: Deep-sea research. Part 1, Oceanographic research papers, Amsterdam [u.a.] : Elsevier Science, 1993, 55(2008), 11, Seite 1590-1599, 1879-0119
    In: volume:55
    In: year:2008
    In: number:11
    In: pages:1590-1599
    Description / Table of Contents: Free or hydrate-bound gas in the seafloor has been of scientific, ecologic and economic interest for many years because it predominantly contains high concentrations of low-molecular-weight hydrocarbons. A prerequisite of accurate quantifications of gases in sediments is to preserve pressure and temperature close to the in situ conditions during recovery. Here we introduce two new sediment coring devices that allow for the recovery of near-surface gas- and gas-hydrate-bearing sediments and subsequent investigations using several different techniques such as visualisation by computerized tomography, quantitative degassing, and sediment and porewater analyses. The first coring tool, the Multiple Autoclave Corer (MAC), resembles a standard multiple corer in terms of applications, size and core length of about 55 cm. The second tool, the Dynamic Autoclave Piston Corer (DAPC), is similar to a piston corer in application and size and enables one to take cores of up to 2.5 m length. Both focus on the investigation of near-surface sediments, which are most strongly affected by changes in bottom-water temperature and hydrostatic pressure, which in turn influence continental slope stability. Some results from recent offshore applications show the potential of these tools.
    Type of Medium: Online Resource
    Pages: Ill., graph. Darst
    ISSN: 1879-0119
    Language: English
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  • 6
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2017-06-27
    Description: The bacterial strain Gp_4_7.1T, isolated from the marine sponge Isops phlegraei collected at the Sula Ridge off the Norwegian coast, was characterized. The isolate was a motile spirillum that was monopolarly and monotrichously flagellated. It was aerobic, Gram-negative, oxidase-positive and catalase-negative. Optimal growth occurred between 20 and 30 °C, at pH 7–8 and with a salt concentration of 2–3 % (w/v). The isolate showed a relatively restricted nutritional profile. Substrate utilization tests were only positive for arabinose. Enzyme tests were positive for esterase lipase C8, lipase C14, leucine arylamidase and naphthol-AS-BI-phosphohydrolase. The strain was not able to reduce nitrate. The major cellular fatty acids were C16 : 1 ω7 and C16 : 0. The DNA G+C content was 62.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison classified the strain as a member of the order Oceanospirillales in the class Gammaproteobacteria. Strain Gp_4_7.1T formed a distinct phyletic line with less than 94 % 16S rRNA gene sequence similarity to its closest relatives with validly published names. Based on the determined data, it is proposed that the strain represents a novel species in a new genus, Spongiispira norvegica gen. nov., sp. nov.; the type strain of Spongiispira norvegica is Gp_4_7.1T (=DSM 17749T =NCIMB 14401T).
    Type: Article , PeerReviewed
    Format: text
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  • 8
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    American Society for Microbiology
    In:  Applied and Environmental Microbiology, 71 (8). pp. 4345-4351.
    Publication Date: 2017-07-07
    Description: The anaerobic oxidation of methane (AOM) is a key process in the global methane cycle, and the majority of methane formed in marine sediments is oxidized in this way. Here we present results of an in vitro 13CH4 labeling study (δ13CH4, ∼5,400‰) in which microorganisms that perform AOM in a microbial mat from the Black Sea were used. During 316 days of incubation, the 13C uptake into the mat biomass increased steadily, and there were remarkable differences for individual bacterial and archaeal lipid compounds. The greatest shifts were observed for bacterial fatty acids (e.g., hexadec-11-enoic acid [16:1Δ11]; difference between the δ13C at the start and the end of the experiment [Δδ13Cstart-end], ∼160‰). In contrast, bacterial glycerol diethers exhibited only slight changes in δ13C (Δδ13Cstart-end, ∼10‰). Differences were also found for individual archaeal lipids. Relatively high uptake of methane-derived carbon was observed for archaeol (Δδ13Cstart-end, ∼25‰), a monounsaturated archaeol, and biphytanes, whereas for sn-2-hydroxyarchaeol there was considerably less change in the δ13C (Δδ13Cstart-end, ∼2‰). Moreover, an increase in the uptake of 13C for compounds with a higher number of double bonds within a suite of polyunsaturated 2,6,10,15,19-pentamethyleicosenes indicated that in methanotrophic archaea there is a biosynthetic pathway similar to that proposed for methanogenic archaea. The presence of group-specific biomarkers (for ANME-1 and ANME-2 associations) and the observation that there were differences in 13C uptake into specific lipid compounds confirmed that multiple phylogenetically distinct microorganisms participate to various extents in biomass formation linked to AOM. However, the greater 13C uptake into the lipids of the sulfate-reducing bacteria (SRB) than into the lipids of archaea supports the hypothesis that there is autotrophic growth of SRB on small methane-derived carbon compounds supplied by the methane oxidizers.
    Type: Article , PeerReviewed
    Format: text
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  • 9
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    National Academy of Sciences
    In:  PNAS Proceedings of the National Academy of Sciences of the United States of America, 101 (30). pp. 11111-11116.
    Publication Date: 2017-06-28
    Description: The anaerobic oxidation of methane (AOM) is one of the major sinks of this substantial greenhouse gas in marine environments. Recent investigations have shown that diverse communities of anaerobic archaea and sulfate-reducing bacteria are involved in AOM. Most of the relevant archaea are assigned to two distinct phylogenetic clusters, ANME-1 and ANME-2. A suite of specific 13C-depleted lipids demonstrating the presence of consortia mediating AOM in fossil and recent environments has been established. Here we report on substantial differences in the lipid composition of microbial consortia sampled from distinct compartments of AOM-driven carbonate reefs growing in the northwestern Black Sea. Communities in which the dominant archaea are from the ANME-1 cluster yield internally cyclized tetraether lipids typical of thermophiles. Those in which ANME-2 archaea are dominant yield sn-2-hydroxyarchaeol accompanied by crocetane and crocetenes. The bacterial lipids from these communities are also distinct even though the sulfate-reducing bacteria all belong to the Desulfosarcina/Desulfococcus group. Nonisoprenoidal glycerol diethers are predominantly associated with ANME-1-dominated communities. Communities with ANME-2 yield mainly conventional, ester-linked diglycerides. ANME-1 archaea and associated sulfate-reducing bacteria seem to be enabled to use low concentrations of methane and to grow within a broad range of temperatures. Our results offer a tool for the study of recent and especially of fossil methane environments.
    Type: Article , PeerReviewed
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  • 10
    Publication Date: 2017-06-27
    Description: Strain HAL40bT was isolated from the marine sponge Haliclona sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. It was aerobic, Gram-negative and oxidase- and catalase-positive. Optimal growth was observed at 20–30 °C, pH 7–9 and in 3 % NaCl. Substrate utilization tests were positive for arabinose, Tween 40 and Tween 80. Enzyme tests were positive for alkaline phosphatase, esterase lipase (C8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N-acetyl-β-glucosaminidase. The predominant cellular fatty acid was C17 : 1 ω8, followed by C17 : 0 and C18 : 1 ω7. Analysis by matrix-assisted laser desorption/ionization time-of-flight MS was used to characterize the strain, producing a characteristic low-molecular-mass protein pattern that could be used as a fingerprint for identification of members of this species. The DNA G+C content was 69.1 mol%. Phylogenetic analysis supported by 16S rRNA gene sequence comparison classified the strain as a member of the class Gammaproteobacteria. Strain HAL40bT was only distantly related to other marine bacteria including Neptunomonas naphthovorans and Marinobacter daepoensis (type strain sequence similarity 〉90 %). Based on its phenotypic, physiological and phylogenetic characteristics, it is proposed that the strain should be placed into a new genus as a representative of a novel species, Spongiibacter marinus gen. nov., sp. nov.; the type strain of Spongiibacter marinus is HAL40bT (=DSM 17750T =CCUG 54896T).
    Type: Article , PeerReviewed
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