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  • 1
    Online Resource
    Online Resource
    Cham :Springer International Publishing AG,
    Keywords: Mass spectrometry. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (128 pages)
    Edition: 1st ed.
    ISBN: 9783319012520
    Series Statement: Lecture Notes in Chemistry Series ; v.83
    Language: English
    Note: Intro -- Foreword -- Acknowledgments -- Contents -- 1 Spectroscopy and the Electromagnetic Spectrum -- 1.1…Light and Matter -- 1.2…The Nature of Light -- 1.3…Vibrations -- 1.3.1 Background -- 1.3.2 Biomolecular Vibrations -- 1.4…Electronic Transitions -- 1.4.1 Background -- 1.4.2 Applications to Biomolecular Systems -- 1.5…Absorption Spectroscopy -- 1.6…Consequence Spectroscopy -- 1.6.1 Background -- 1.6.2 Infrared Multiple-Photon Dissociation Spectroscopy -- 1.6.3 Ultraviolet Photodissociation Spectroscopy -- 1.6.4 Infrared--Ultraviolet Ion Dip Spectroscopy -- 1.6.5 Infrared Predissociation Spectroscopy -- References -- 2 Light Sources -- 2.1…Laser Theory -- 2.1.1 Light Sources Employed in Photodissociation Experiments -- 2.1.2 Necessity of High-Intensity Light Sources -- 2.1.3 Amplification by Stimulated Emission -- 2.1.4 Population Inversion -- 2.1.5 3- and 4-Level Systems -- 2.1.6 Continuous Mode and Pulsed Mode (Q-Switching) -- 2.1.7 Cavities and Tuning -- 2.2…Gas Lasers -- 2.2.1 The CO2 Gas Discharge Laser -- 2.2.2 Rotational States and Rovibrational Transitions of CO2 -- 2.2.3 Excimer Lasers and Other VUV Lasers -- 2.3…Solid-State Lasers -- 2.3.1 Nd:YAG -- 2.3.2 Widely Tunable Solid-State Lasers -- 2.4…Nonlinear Optics Light Sources -- 2.4.1 Optical Parametric Oscillators/Amplifiers -- 2.4.2 Nonlinear Crystals -- 2.5…Dye Lasers -- 2.5.1 Dye Laser Theory -- 2.5.2 Dyes Used -- 2.6…Semiconductor Lasers -- 2.6.1 Semiconductor Basics -- 2.6.2 Laser Diode Operation -- 2.6.3 Quantum Cascade Laser Operation -- 2.7…Other Light Sources -- 2.8…Concluding Remarks -- References -- 3 Ion Traps -- 3.1…Introduction -- 3.2…Penning TrapPenning traps -- 3.2.1 Basic Equations -- 3.2.2 Ion Excitation and Detection of the Signal -- 3.3…RF Traps -- 3.3.1 2D Quadrupole Ion TrapQuadrupole Ion Trap (Linear Ion Trap). , 3.3.1.1 The Equations of Motion in a Quadrupolar Field -- 3.3.1.2 Ion Motion in the 2D Trap and Capturing Ions in Three Dimensions -- 3.3.1.3 Mass Selectivity as Linear Mass Filter -- 3.3.2 3D Quadrupole Ion Trapquadrupole on trap -- 3.3.2.1 The Equations of Motion in a 3D QIT -- 3.3.2.2 Quadrupole Ion Trap as Mass Analyzer -- 3.3.3 Multipole Devices -- 3.4…Cold Trapcold traps -- 3.5…Comparison of Traps -- References -- 4 Infrared Photodissociation of Biomolecular Ions -- 4.1…Infrared Photodissociation Spectra of Mass-separated Biomolecular Ions -- 4.2…Photodissociation with CO2 Lasers -- 4.2.1 Background -- 4.2.2 Peptides -- 4.2.3 Saccharides -- 4.3…Photodissociation with Optical Parametric Oscillators -- 4.3.1 Background -- 4.3.2 Peptides -- 4.3.3 Saccharides -- 4.4…IR spectroscopy of Cold Ions -- 4.5…Longer-Term Considerations for IR Spectroscopy of Mass-separated Biomolecules -- 4.5.1 Current Constraints -- 4.5.2 Future Directions -- References -- 5 UV-Visible Activation of Biomolecular Ions -- 5.1…Electronic Excitation of Biomolecular Ions -- 5.2…Gas-Phase Visible and Near-UV Excitation of Biomolecular Ions -- 5.3…UV Photodissociation of Protonated Peptides -- 5.3.1 Relaxation and FragmentationFragmentation of Electronic Excited Peptides: The Example of Leucine-Enkephalin -- 5.3.2 Peptide SequencingSequencing with UVPD -- 5.4…Photoinduced Radical Chemistry -- 5.4.1 Background -- 5.4.2 UV Photodissociation of Phosphopeptides -- 5.4.3 Activated-Electron Photodetachment Dissociation -- 5.5…Coupling of UV-Visible FragmentationFragmentation with Separation Methods -- 5.5.1 Coupling of Photodissociation with Liquid Chromatography -- 5.5.2 Coupling of Photodissociation with Ion Mobility Spectrometry -- 5.6…Photodissociation of Biomolecular Ions with Ultrashort Pulses -- 5.6.1 Femtosecond LaserLaser-Induced Ionization/Dissociation of Peptides. , 5.6.2 Femtosecond Pump-Probe Experiments on Trapped Flavin: Optical Control of Dissociation -- 5.7…Future Directions -- References -- Index.
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  • 2
    Electronic Resource
    Electronic Resource
    350 Main Street , Malden , MA 02148 , USA , and 9600 Garsington Road , Oxford OX4 2DQ , UK . : Blackwell Publishing, Inc.
    Risk analysis 23 (2003), S. 0 
    ISSN: 1539-6924
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: In this work we characterize risk perception in Chile, based on the psychometric paradigm, exploring the difference between perceived social and personal risk. For this purpose, we conducted a survey including 54 hazards, 16 risk attributes, and 3 risk constructs. The survey, divided into four parts, was administered to 508 residents of Santiago, Chile. Using factor analysis, three main factors, which accounted for 80% of the sample's variance, were identified: factor 1, commonly called “Dread Risk” in the literature, explained 37% of variance; factor 2, “Unknown Risk,” explained 28%; and factor 3, which we called “Personal Effect,” explained 15% of the variance. On average, individuals perceived themselves as less exposed to risk and with more control and knowledge about them than the general population. OLS regression models were used to test the association of perceived risk with the three main factors. For social risk, factor 1 had the greatest explanatory power, while factor 2 had a negative sign. For personal risk, only factors 2 and 3 were significant, with factor 3 having the greatest explanatory power. Risk denial (defined as the difference between perceived personal and social risk) was associated with factors 1 and 2 only, with factor 2 having a negative sign. The difference between desired and actual regulation levels proved positive for all hazards, thus indicating that Chileans are dissatisfied with the current regulation level for all the hazards analyzed. The comparison of data at the aggregate and at the individual subject's level suggests that while the aggregate analysis overestimates the magnitude of the correlations it still reflects the tendency of the individual responses.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We have studied the regional distribution and characteristics of polyamine-sensitive [3H]ifenprodil binding sites by quantitative autoradiography in the rat brain. In forebrain areas ifenprodil displaced [3H]ifenprodil (40 nM) in a biphasic manner with IC50 values ranging from 42 to 352 nM and 401 to 974 µM. In hindbrain regions, including the cerebellum, ifenprodil displacement curves were monophasic with IC50 values in the high micromolar range. Wiping studies using forebrain slices (containing both high- and low-affinity sites) or cerebellar slices (containing only the low-affinity site) showed that high- and low-affinity ifenprodil sites are sensitive to spermine and spermidine, to the aminoglycoside antibiotics neomycin, gentamicin, and kanamycin, and to zinc. Two calmodulin antagonists, W7 and calmidazolium, also displaced [3H]ifenprodil from both sites. Other calmodulin antagonists, including trifluoperazine, prenylamine, and chlorpromazine, selectively displaced [3H]ifenprodil from its low-affinity site in hindbrain and forebrain regions. High-affinity [3H]ifenprodil sites, defined either by ifenprodil displacement curves or by [3H]ifenprodil binding in the presence of 1 mM trifluoperazine, were concentrated in the cortex, hippocampus, striatum, and thalamus with little or no labeling of hindbrain or cerebellar regions. This distribution matches that of NMDAR2B mRNA, supporting data showing that ifenprodil has a preferential action at NMDA receptors containing this subunit. Low-affinity [3H]ifenprodil sites have a more ubiquitous distribution but are especially concentrated in the molecular layer of the cerebellum. [3H]Ifenprodil was found to bind to calmodulin-agarose with very low affinity (IC50 of ifenprodil = 516 µM). This binding was displaced by calmodulin antagonists and by polyamines, with a potency that matched their displacement of [3H]ifenprodil from its low-affinity site in brain sections. However, the localization of the low-affinity [3H]ifenprodil site does not strictly correspond to that of calmodulin, and its identity remains to be further characterized. The restricted localization of high-affinity [3H]ifenprodil binding sites to regions rich in NMDAR2B subunit mRNA may explain the atypical nature of this NMDA antagonist.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 62 (1994), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We report here a photoneural regulation of nitric oxide synthase (NOS) activity in the rat pineal gland. In the absence of the adrenergic stimulation following constant light exposure (LL) or denervation, pineal NOS activity is markedly reduced. A maximal drop is measured after 8 days in LL. When rats are housed back in normal light-dark (LD) conditions (12:12), pineal NOS activity returns to normal after 4 days. A partial decrease in pineal NOS activity is also observed when rats are placed for 8 days in LD 18:6 or shorter dark phases, indicating that pineal NOS activity reflects the length of the dark phase. Because it is known that norepinephrine (NE) is released at night from the nerve endings in the pineal gland and this release is blocked by exposure to light, our data suggest that NOS is controlled by adrenergic mechanisms. Our observation may also explain the lack of cyclic GMP response to NE observed in animals housed in constant light.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 60 (1993), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Pineal arylalkylamine N-acetyltransferase (N-acetyltransferase) controls large daily changes in melatonin production. It is generally thought that the activity of this enzyme is controlled by norepinephrine acting exclusively via elevation of cyclic AMP. However, norepinephrine also elevates pineal intracellular Ca2+ concentration ([Ca2+]i), and it is not known whether Ca2+ is involved in regulating N-acetyltransferase activity other than through its established role in cyclic AMP production. In this study, the issue of whether Ca2+ enhances the effects of cyclic AMP on N-acetyltransferase activity was investigated. The effects of cyclic AMP protagonists (isobutylmethylxanthine, N6, 2′-O-dibutyryladenosine 3′,5′-cyclic monophosphate, 8-bromoadenosine 3′,5′-cyclic monophosphate, and adenosine 3′,5′-cyclic monophosphothioate, Sp-diastereomer) were examined in combination with [Ca2+]i protagonists (A23187, ionomycin, and phenylephrine). All [Ca2+]i protagonists potentiated the effects of cyclic AMP protagonists. For example, ionomycin potentiated the effects of low concentrations of 8-bromoadenosine 3′,5′-cyclic monophosphate, and A23187 potentiated the effects of isobutylmethylxanthine without altering cyclic AMP accumulation. These findings indicate that Ca2+ and cyclic AMP probably act physiologically in a coordinated manner to stimulate N-acetyltransferase activity; these second messengers could act directly at one or more sites or through indirect actions mediated by kinases.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 54 (1990), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: In mouse cerebral cortical slices, noradrenaline (NA) potentiates cyclic AMP (cAMP) accumulation elicited by vasoactive intestinal peptide (VIP) through α1-adrenergic receptors. This synergism is inhibited by indomethacin, and the prostaglandins E2 and F2α mimic the effect of NA. In the present study, we observed that the synergism between VIP and NA is not inhibited by the protein kinase C inhibitor 1-(5-isoquinolinylsulfonyI)-2-methylpiperazine (H-7) or the diacylglycerol-lipase inhibitor RHC 80267, thus further stressing the role of phospholipase A2 activation. Various neuroactive agents that potentiate the stimulatory effect of VIP on cAMP formation were also examined. As with NA, the potentiation by histamine and adenosine is inhibited by indomethacin. In contrast to NA, histamine, and adenosine, the synergistic interaction between phorbol esters and VIP on cAMP formation is abolished by H-7 but not by indomethacin. The potentiation by baclofen, a y-aminobutyric acidB receptor agonist, is partially inhibited by the 5-lipox-ygenase inhibitor nafazatrom. The synergism between ouabain and VIP is reduced by H-7 but not by indomethacin and nafazatrom. These data indicate that the stimulation of cAMP formation elicited by VIP is under the modulation of various neuroactive agents that trigger diverse intracellular mechanisms to potentiate the effect of the peptide.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 65 (1995), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We have previously shown that exposure of rats to constant light (LL) induced a decrease in NO synthase (NOS) activity in the pineal gland. We present here the evidence that chronic (5 days) norepinephrine (NE) or isoproterenol treatment prevents the effect of LL and enhances pineal NOS activity in LL animals. This effect of NE appears to be mediated by β-adrenoceptors, because it was not mimicked by the α-agonist phenylephrine. Pineal NOS activity was reduced in 16-h light/8-h dark animals treated for 4 days with the β-adrenergic antagonist propranolol but not with the α1-antagonist prazosin, indicating again an involvement of β-adrenergic receptor in the control of NOS. Treatment with adrenergic antagonists did not affect cortical NOS activity, suggesting that the control of NOS is different in these two tissues or that the pineal expresses a specific isoform of the enzyme. Taken together, these data suggest that NE controls NOS in the pineal gland through β-adrenergic receptors. To our knowledge, this represent the first demonstration of a regulation of NOS by a neurotransmitter in the CNS, as assayed under Vmax conditions.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: KCI (20–100 mM) and W-methyl-D-aspartate (NMDA, 100–1,000 μM) produce concomitant concentration-dependent increases in the release of previously captured [14C]acetylcholine and [3H]spermidine from rat striatal slices in vitro. The effects of NMDA (300μM) on striatal [14C]acetylcholine and [3H]spermidine release were blocked with equal potencies by the competitive NMDA antagonist CGP 37849, the glycine site antagonist L-689,560, and the NMDA channel blocker dizocilpine. In contrast, although NMDA-evoked [14C]acetylcholine release was antagonized by ifenprodil (IC50= 5.3 μM) and MgCl2, (IC50= 200 μM), neither compound antagonized the NMDA-evoked release of [3H]spermidine at concentrations up to 100 μM (ifenprodil) or 1 mM (MgCl2). Distinct NMDA receptor subtypes with different sensitivities to magnesium and ifenprodil therefore exist in the rat striaturn.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Addiction 100 (2005), S. 0 
    ISSN: 1360-0443
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Psychology
    Notes: Aims  To examine indicators of buprenorphine diversion and injection among injecting drug users in Melbourne, Australia and to determine the factors associated with buprenorphine injection.Design  Melbourne arm of the 2002 Illicit Drug Reporting System (IDRS) cross-sectional study.Setting  Five Needle and Syringe Programme sites in Melbourne, Australia.Participants  A total of 156 current injecting drug users (IDU). Study elibility criteria were at least monthly injection during the previous 6 months, and Melbourne residence for at least the preceding 12 months.Measurements  Structured questionnaire covering demographic characteristics, drug use history, the price, purity and availability of drugs, criminal activity, risk-taking behaviours, health-related issues and general drug use trends.Findings  Over one-third (37%) of the study sample reported injecting buprenorphine in their life-time and 33% reported injecting the drug in the last 6 months. Recent buprenorphine injection was associated with the injection of other drug types (i.e. polydrug injectors), opioid substitution treatment, injection-related health problems and involvement in crime. Almost half (47%) of those who reported recent buprenorphine injection reported obtaining the drug illicitly at least once during that time.Conclusions  Given the significant health harms associated with intravenous buprenorphine use (e.g. vein damage, abscesses and infections, precipitated withdrawal, blood-borne virus transmission, hospitalization and death), routine monitoring of the misuse of buprenorphine in Melbourne is warranted. These results suggest the need for development of effective countermeasures to address diversion and injection of buprenorphine in this setting.
    Type of Medium: Electronic Resource
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