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  • 1
    Electronic Resource
    Electronic Resource
    Expression of mucin type carbohydrates may supplement histologic diagnosis in oral premalignant lesions (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 20 (1991), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Recent studies have shown that changes within membrane bound carbohydrates may be essential for cellular differentiation and malignant transformation. We have therefore, by means of immunohistochemistry, studied the expression of T/Tn related (Thomsen-Friedenrich) carbohydrates in 13 oral lesions with squamous cell dysplasia. The epithelial grade of dysplasia was graded as mild, moderate or severe. The following carbohydrate structures were studied: Tn, T, mucintype 3 chain H, and the sialylated derivates. sialosyl-Tn and sialosyl-T. In general, short structures were detected on the basal cells and longer structures on the more mature spinous cells. In many cases, this sequential expression was more disturbed with increasing grade of epithelial dysplasia. However, our results also showed that some lesions with the same grade of epithelial dysplasia showed different carbohydrate expression. These findings indicate that expression of carbohydrates may supplement histologic diagnosis in the evaluation of the prognosis of premalignant lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1991.tb00904.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the histo-blood group ABO gene defined glycosyltransferases in epithelial tissues (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 19 (1990), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The histo-blood group ABO carbohydrate antigens are differentially expressed in epithelia in close correlation with cellular differentiation. In order to gain insight into the biosynthetic regulation of these carbohydrate antigens, we correlated the expression of A carbohydrate antigens with that of the A gene defined glycosyl-transferase by immunohistology of human oral epithelia using monoclonal antibodies. In glandular epithelium the A transferase was found in mucous cells similar to that of the A carbohydrate antigens. In stratified non-keratinized squamous epithelium the A transferase was expressed only in spinous cell layers, which is in accordance with the appearance of the A carbohydrate antigens in these more mature cell layers. This simultaneous acquisition of the primary and secondary gene product of a glycosyltransferase gene, provides evidence that the well-defined sequential expression of histo-blood group carbohydrate antigens in stratified squamous epithelium may be directly regulated at the transcriptional level of the glycosyltransferase. Future studies will address the mechanism behind loss of A antigens in premalignant lesions and carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1990.tb00836.x
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  • 3
    Electronic Resource
    Electronic Resource
    Blood group A/B-defined glycosyltransferase and A/B blood group antigens in human normal and malignant endometrium in relation to morphology, age and oestrogen levels (1994)
    Springer
    Glycoconjugate journal 1 (1994), S. 153-163 
    Details
    ISSN: 1573-4986
    Keywords: A/B gene-defined transferase ; blood grup ABO carbohydrate antigens ; endometrial carcinoma ; human endometrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract We have used monoclonal antibodies to study the expression and regulation of A/B antigens and A/B transferase in normal and malignant human endometrium by immunohistochemistry. Staining was evaluated against blood group status, morphology, age ad serum oestrogen levels. The expression of the antigens, in contrast tothe expression of the transferase, was related to the A subtype (A1/A2) and the ABH secretor status. Normal, non-secretory endometria and most well-differentiated endometrial carcinomas from ABH secretors expressed the antigens and the transferase, but showed a morphology-dependent variation in the expression and degree of coexpression. n contrast, most grade 2 and 3 carcinomas were found to lack both structures, whereas secretory endometrium had a high expression of the transferase but expressed the antigens on only a few cells. The transferase expression was correlated inversely with age and positively with the level of free oestradiol in serum. Our findings suggest that A/B antigenic expression in the endometrium may be regulated at different levels — at the A/B transferase level and at a precursor substrate lvel — and that both genetic and hormonal factors are probably involved in the regulatory process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902188
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  • 4
    Electronic Resource
    Electronic Resource
    N-acetyllactosamine and sialosyl-N-acetyllactosamine in normal and malignant human endometrium (1994)
    Springer
    Glycoconjugate journal 1 (1994), S. 271-278 
    Details
    ISSN: 1573-4986
    Keywords: N-acetyllactosamine ; endometrium ; human ; sialylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract We have evaluated using immunohistochemistry the expression of the type 2 chain histo-blood group precursorsN-acetyllactosamine (Lac), sialosyl-Lac (S-Lac) and binary-sialosyl Lac (DS-Lac) in epithelial cells of normal non-secretory, gestational and malignant human endometrial tissues (n=120). Staining was assessed in relation to genetic (ABO, Lewis blood group and secretor status), morphologic and hormonal factors (serum levels of estrogens). The staining pattern for Lac, S-Lac and DS-Lac showed great variation and was not related to blood group or the secretor status. Staining for Lac showed a limited distribution in both normal and malignant endometrium and was most frequenly found in gestational and atrophic endometrium. S-Lac was strongly expressed, but only infrequently as DS-Lac structures in normal endometrium. Staining for both S-Lac and DS-Lac was most widespread in proliferating endometria. Endometrial carcinomas showed an increased staining for DS-Lac and a varied, and in most cases a reduced, staining for S-Lac, a pattern like that previously found in secretory endometrium. Staining scores for S-Lac showed a weak correlation with serum levels of free estradiol. Thus, the increased expression of DS-Lac in contrast to S-Lac structures in endometrial carcinomas is probably unrelated to both hormonal and genetic factors and may be considered a ‘tumor-associated’ but not a tumor-specific change in endometrial cell glycosylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00919335
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  • 5
    Electronic Resource
    Electronic Resource
    Development and characterization of an antibody directed to an α-N-acetyl-D-galactosamine glycosylated MUC2 peptide (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 51-62 
    Details
    ISSN: 1573-4986
    Keywords: Tn-antigen ; MUC2 ; mucin ; glycopeptide ; carbohydrate antigen ; O-glycosylation ; monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In an attempt to raise anti-Tn antibodies, an α-N-acetyl-D-galactosamine glycosylated peptide based on the tandem repeat of the intestinal mucin MUC2 was used as an immunogen. The MUC2 peptide (PTTTPISTTTMVTPTPTPTC) was glycosylated in vitro using concentrated α-N-acetylgalactosaminyltransferases activity from porcine submaxillary glands which resulted in the incorporation of 8–9 mol of Ga/NAc. Rabbits and mice developed specific anti-MUC2-GalNAc glycopeptide antibodies and no detectable anti-Tn antibodies. Anti-glycopeptide antibodies did not show reactivity with the unglycosylated MUC2 peptide or with other GalNAc glycosylated peptides. A mouse monoclonal antibody (PMH1) representative of the observed immune response was generated and its immunohistological reactivity analysed in normal tissues. PMH1 reacted similarly to other anti-MUC2 peptide antibodies. However, in some cells the staining was not restricted to the supranuclear area but extended to the entire cytoplasm. In addition, PMH1 reacted with purified colonic mucin by Western blot analysis suggesting that PMH1 reacted with some glycoforms of MUC2. The present work presents a useful approach for development of anti-mucin antibodies directed to different glycoforms of individual mucins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006939432665
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of capillaries in sections from skeletal muscle by use of lectins and monoclonal antibodies reacting with histo-blood group ABH antigens (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 181-188 
    Details
    ISSN: 1573-4986
    Keywords: muscle capillaries ; blood group carbohydrate antigens ; lectins ; monoclonal antibodies ; histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract This study was performed to evaluate the application of different lectins and monoclonal antibodies against ABH antigens to detect and characterize carbohydrate structures in capillaries of skeletal muscle from humans and laboratory animals. Blood group specific lectins (Griffonia simplicifolia, Griffonia simplicifolia isolectin B4,Lotus tetragonlobus, Ulex europaeus, andDolichos biflorus) and monoclonal antibodies reacting with histo-blood group carbohydrate antigens belonging to type 1 (Lea) and type 2 (H, A and Ley) chains were used as histological markers for capillaries in sections from skeletal muscle. The material consisted of 20 human masseter muscle biopsies from individuals with known blood types: (eight blood group O, nine blood group A, two blood group B, and one blood group AB) and masseter muscles specimens from different laboratory animals (mouse, rat, rabbit, cat, dog, pig, cow, and macaca monkey). Unfixed sections and an avidin alkaline phosphatase method were used to visualize the specific reaction.Ulex lectin stained capillaries in all human biopsies either strongly or moderately. Strong muscle capillary reaction was observed in biopsies from O, B and AB individuals while capillaries from A individuals were only moderately stained.Griffonia simplicifolia marked capillaries in A, B, and AB individuals andGriffonia simplicifolia isolectin B4 stained capillaries in muscle biopsies from B and AB donors.Dolichos biflorus was a weak marker of muscle capillaries from A individuals. Only capillaries from O individuals were stained with the antibody against H type 2. Capillary reaction was not observed with the other antibodies used.Girffonia simplicifolia was an excellent marker for capillaries in mouse muscle whileGriffonia simplicifolia isolectin B4 is recommended for rat muscles. Periodic acid treatment and subsequentLotus tetragonolobus staining is suitable to visualize capillaries in mouse, rat and pig muscle. Using a sensitive histochemical technique for staining with lectins and monoclonal antibodies reacting with blood group related antigens the microvascular density in human skeletal muscle may be estimated. Further, the carbohydrate compounds in the muscle capillaries reflect the individual blood type. A selection of lectins is suitable for demonstration of capillaries in animal skeletal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00737716
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  • 7
    Electronic Resource
    Electronic Resource
    The distribution of type-2 chain histo-blood group antigens in normal cycling human endometrium (1992)
    Springer
    Cell & tissue research 270 (1992), S. 425-433 
    Details
    ISSN: 1432-0878
    Keywords: Human cycling endometrium ; Type-2 chain ABO antigens ; Immunohistochemistry ; Genetic and hormonal regulation ; Genetic regulation ; Hormonal regulation ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The blood group ABO(H) determinants are major allogenic antigens in both erythrocytes and tissue of man. These antigens and related carbohydrates are markers of cellular maturation and differentiation in many epithelial tissues and have recently attracted great interest as tumor-associated antigens. Previous studies of endometrial tissues have indicated that glycosylation in this tissue may be related to hormonal stimulation. We have investigated the immunohistochemical distribution of type-2 chain histo-blood group-related carbohydrates in specimens of normal, cycling endometria obtained from hysterectomies on women with known ABO/Lewis erythrocyte type and saliva secretor status. N-acetyllactosamine and Lex were demonstrated to be uninfluenced by the genetic background. A and Aley antigens were exclusively demonstrated in endometria from blood group A individuals, while Ley was expressed in endometria from blood group 0 individuals mainly. The precursor N-acetyllactosamine as well as the terminal H, A, and ALey antigens were shown in only a few cells. In contrast, N-acetyllactosamine substituted by sialic acid and/or fucose residues (Lex, sialosyl-Lex, Ley) were demonstrated in epithelial cells of normal, cycling endometrium, but with both quantitative and qualitative differences in staining relating to the menstrual cycle, indicating that type-2 chain antigens are expressed under both genetic and hormonal influence in human cycling endometrium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00645043
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