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  • 1
    Electronic Resource
    Electronic Resource
    Development of EDTA Media for the Rapid Growth of Chlamydomonas mundana (1963)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 10 (1963), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. An empirical method is used to determine mixtures of the chelating agent, EDTA (ethylenediamine tetraacetate), and trace metals which allow maximum growth rates of Chlamydomonas mundana under optimum environmental conditions. In this method which resembles an immunological titration one trace metal (Ca, Fe, Mn or Zn), in increasing concentrations, is used to “saturate” a standard amount of EDTA. Other required trace metals are added in a range of minimum concentrations. Extent of growth with each mixture is evaluated after 24 hours of incubation. Mixtures allowing greatest growth are then used to evaluate growth rate. Media with manganese as the EDTA-saturating trace metal allow most rapid growth rates equivalent to 11.0 doublings per day. Magnesium as the EDTA-saturating metal does not allow growth, suggesting that Mg does not displace other required trace metals from EDTA-complex. Since the mixtures allowing maximum growth rate are “underchelated” it appears that the free metals rather than the metal-chelate complexes are utilized by the alga in rapid growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1963.tb01670.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of pH of the Medium on the Availability of Chelated Iron for Chlamydomonas mundana (1965)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 12 (1965), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Nutritional and cultural factors influenced growth of Chlamydomonas mundana at different H-ion concentrations. Growth at acid pH (5.5–6.0) primarily depended on the acetate and chelated iron concentration and on the method of preparation of the medium. The organism had increased Na, Ca, and Mn requirements at an acid pH. Sensitivity to increased levels of phosphate was greater at low than at high pH. The requirement for chelated as compared with non-chelated Fe was qualitatively easier to demonstrate at low pH. The procedure followed in preparing the growth media was more important with higher levels of chelator and Fe and with media of low pH. All 6 synthetic chelators studied were more effective, as measured by growth, if combined with iron before addition to the media. At high pH (7.0–7.5), the organism was much more adept at utilizing over-chelated Fe. This latter finding, and the greater sensitivity at low pH to trace-metal imbalance in ill-prepared media, indicate that the organism absorbs Fe much more efficiently at neutral or slightly alkaline pH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1965.tb03248.x
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    Paper (German National Licenses)
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