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  • 1
    Electronic Resource
    Electronic Resource
    Purification and characterization of a monomeric isocitrate dehydrogenase from the sulfate-reducing bacterium Desulfobacter vibrioformis and demonstration of the presence of a monomeric enzyme in other bacteria (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 160 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: NADP+-specific isocitrate dehydrogenase (EC 1.1.1.42) was purified to homogeneity from the sulfate-reducing bacterium Desulfobacter vibrioformis, and shown to be a monomeric protein with a molecular mass of 80 kDa. The pH and temperature optima were 8.5 and 45°C, respectively. The N-terminal amino acid sequence (Thr, Glu, Thr, Ile, Arg, Trp, Thr, X, Thr, Asp, Glu, Ala, Pro, Leu, Leu, Ala, Thr) showed similarity with that of other known monomeric isocitrate dehydrogenases. Catalytically active isocitrate dehydrogenase from D. vibrioformis was obtained by activity staining after SDS-PAGE and removal of SDS from the gel. This technique revealed a NADP+-dependent monomeric enzyme in other Desulfobacter spp., Desulfuromonas acetoxidans and Chlorobium tepidium. These findings imply that monomeric isocitrate dehydrogenases are present in distantly related bacteria and indicate an early evolution of monomeric isocitrate dehydrogenases in the bacterial lineage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb12893.x
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  • 2
    Electronic Resource
    Electronic Resource
    Corrigendum to ‘Purification and characterization of a monomeric isocitrate dehydrogenase from the sulfate-reducing bacterium Desulfobacter vibrioformis and demonstration of the presence of a monomeric enzyme in other bacteria’ (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 161 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb12970.x
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  • 3
    Electronic Resource
    Electronic Resource
    Synchronous Cultures of Chlamydomonas reinhardti: Properties and Regulation of Repressible Phosphatases (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 28 (1973), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: De novo synthesis of phosphatase (derepression) in orthophosphate deprived synchronously growing Chlamydomonas reinhardti has been demonstrated by using a double labelling isotope technique coupled with cellulose acetate electrophoresis. Repressed and derepressed phosphatase exhibited different enzymatic properties as pH optimum, electrophoretic pattern, Km and Ki values. Especially the acid phosphatase was located near the cell surface.Inorganic, cold TCA-extractable 32P, decreased during the first 1–2 h after phosphate deprivation when there was little or no net synthesis of phosphatase.Results of experiments with additions of orthophosphate and cycloheximide to derepressed cells, indicated that the derepressible enzyme was relatively unstable, while its m-RNA was relatively stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1973.tb01191.x
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of Indole-3-acetic Acid and Gibberellin on Synchronous Cultures of Chlorella fusca (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 24 (1971), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The applicability of synchronous cultures of Chlorella fusca as a reproducible experimental system for the study of growth regulators has been investigated using indole-3-acetic acid (IAA) and gibberellin.〈list xml:id="l1" style="custom"〉1None of these compounds stimulated growth or sporulation.2IAA inhibited growth and sporulation at concentrations higher than 6 × 10−5M, the effect increasing with increasing acidity. Gibberellin had no effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1971.tb03476.x
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  • 5
    Electronic Resource
    Electronic Resource
    Induction of Isocitrate Lyase in Synchronous Cultures of Chlorella fusca (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 31 (1974), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Isocitrate lyase (ICL) of Chlorella was induced with acetate, and induction kinetics followed in autospores and 6 h old cells of a synchronous culture.The enzyme could not be induced in illuminated cells. With both cell types 1.2 mM acetate was the optimal inducer concentration. Freeze-thawed cells and acetone powders were used for measurement of activity. With the former the time course of increase in activity was different at the two cell ages. With 6 h old cells the activity fluctuated: There was first a period of increase, then one with decrease and again one of increase. No such variation was found with freee-thawed autospores or with acetone powders of both cell stages. Darkening 6 h old cells for different periods of time before induction reduced the peak of activity, leaving the rate of the third phase unchanged. Illumination of darkened cells before induction increased the peak. Increasing the duration of both treatments increased their respective effects.Acetone extracts taken at different times after start of induction inhibited the ICL activity of a test preparation. The inhibition decreased concurrently with the variation in the ICL activity-found-when freee-thawed cells were used in the enzyme assay.The inducibility, taken as the rate of the third phase, was measured at different times during the 24 h synchronous cycle. Using three different acetate concentrations and both methods of cell preparation, we found that the inducibility was constant for 17 h whereafter it increased rapidly to a final level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1974.tb03699.x
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  • 6
    Electronic Resource
    Electronic Resource
    Synchronized cultures of a cell wall-less mutant of Chlamydomonas reinhardii (1976)
    Springer
    Archives of microbiology 108 (1976), S. 189-194 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas reinhardii ; Synchronous growth ; Cell wall-less mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synchronization and synchronous growth of a cell wall-less mutant of Chlamydomonas reinhardii have been described. The following growth conditions were used: A modified Sueokas' “high salt minimal medium”, 14∶10 h light-dark cycle, growth temperature 30°C, light intensity 12–18 Klux and dilution of the culture at the end of the dark to a constant cell density of 1.0·106 cells/ml. The time course of increase and distribution of cell volume, cytoplasmic and nuclear division, release of motile cells after the division period and accumulation of DNA, RNA and protein are reported. These mutant cells did not make any sporangium in which the dividing cells were kept as a unit inside a mother cell wall. However, they usually adhered during the period of division, thus making clumps containing 2, 4 and 8 cells. Several of these cell clumps dissolved releasing either single or couples of 2 and 4 cells. After the end of division the cells became flagellated and motile and thereby releasing themselves from the aggregate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428950
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  • 7
    Electronic Resource
    Electronic Resource
    Degradation and compartmentalization of urea in Chlamydomonas reinhardii (1981)
    Springer
    Archives of microbiology 129 (1981), S. 261-264 
    Details
    ISSN: 1432-072X
    Keywords: Chlamydomonas reinhardii ; Urea ; Compartment ; Ammonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Urea is accumulated against a concentration gradient in Chlamydomonas reinhardii. Only energy generated from photosynthesis is used for this accumulation, while degradation of urea utilizes other energy sources. Exogenous supplied urea is distributed between two pools, one large nonmetabolic and one metabolic pool. Ammonia inhibits the transport from the nonmetabolic to the metabolic pool. The nonmetabolic pool is probably located in the chloroplast, and the accumulation of urea is due to an active transport into the chloroplast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414694
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  • 8
    Electronic Resource
    Electronic Resource
    Variations in the carotenoid content of Chlamydomonas reinhardii throughout the cell cycle (1975)
    Springer
    Archives of microbiology 104 (1975), S. 249-254 
    Details
    ISSN: 1432-072X
    Keywords: Synchronous ; Green Alga ; Volvocales ; Chlamydomonas ; Carotenoid ; Xanthophyll ; Loroxanthin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synchronous cultures of Chlamydomonas reinhardii have been examined for the total amounts of carotenoid and chlorophyll present throughout a 12 hrs light–4 hrs dark life cycle. Variations in the carotenoid distribution at different points within the cell cycle have been found. During the greater part of the light period all major carotenoids increased at a proportionally similar rate. However, the increases in lutein and violaxanthin preceded those in β-carotene and neoxanthin by some 2 hrs and that in loroxanthin, an algal xanthophyll, by about 3 hrs. A marked drop in total carotenoid accumulation, corresponding to similar temporary falling away in the accumulation of β-carotene, lutein and violaxanthin occurred at 9 hrs. The correspondence of this with the established drop in RNA accumulation and the break-up of the nucleolus was pointed out. Considerable redistribution among the carotenoids occurred during the dark period, notably the amount of β-carotene increased relative to the total xanthophylls. The full significance of these results can not be estimated in the absence of comparative data on related organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447333
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  • 9
    Electronic Resource
    Electronic Resource
    Properties and primary structure of a thermostable l-malate dehydrogenase from Archaeoglobus fulgidus (1997)
    Springer
    Archives of microbiology 168 (1997), S. 59-67 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsArchaea ; Archaeoglobus fulgidus ; Thermophiles ; Malate dehydrogenase ; Lactate ; dehydrogenase ; Thermostable protein ; mdh ; Glycine motif ; Lactate-dehydrogenase-like malate ; dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A thermostable l-malate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was isolated and characterized, and its gene was cloned and sequenced. The enzyme is a homodimer with a molecular mass of 70 kDa and catalyzes preferentially the reduction of oxaloacetic acid with NADH. A. fulgidus l-malate dehydrogenase was stable for 5 h at 90° C, and the half-life at 101° C was 80 min. Thus, A. fulgidus l-malate dehydrogenase is the most thermostable l-malate dehydrogenase characterized to date. Addition of K2HPO4 (1 M) increased the thermal stability by 40%. The primary structure shows a high similarity to l-lactate dehydrogenase from Thermotoga maritima and gram-positive bacteria, and to l-malate dehydrogenase from the archaeon Haloarcula marismortui and other l-lactate-dehydrogenase-like l-malate dehydrogenases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050470
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  • 10
    Electronic Resource
    Electronic Resource
    Thermodesulforhabdus norvegicus gen. nov., sp. nov., a novel thermophilic sulfate-reducing bacterium from oil field water (1995)
    Springer
    Archives of microbiology 164 (1995), S. 331-336 
    Details
    ISSN: 1432-072X
    Keywords: Key words Sulfate reduction ; Thermophile ; Oil field water ; Acetate oxidation ; Delta subdivision ; Thermodesulforhabdus norvegicus gen. nov. ; sp. nov
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel gram-negative, thermophilic, acetate-oxidizing, sulfate-reducing bacterium, strain A8444, isolated from hot North Sea oil field water, is described. The rod-shaped cells averaged 1 μm in width and 2.5 μm in length. They were motile by means of a single polar flagellum. Growth was observed between 44 and 74°C, with an optimum at 60°C. Spores were not produced. Sulfate and sulfite were used as electron acceptors. Sulfur, thiosulfate, nitrate, fumarate, and pyruvate were not reduced. In the presence of sulfate, growth was observed with acetate, lactate, pyruvate, butyrate, succinate, malate, fumarate, valerate, caproate, heptanoate, octanoate, nonadecanoate, decanoate, tridecanoate, pentadecanoate, palmitate, heptadecanoate, stearate, and ethanol. Pyruvate, lactate, and fumarate did not support fermentative growth. Cytochromes of the c-type were present. Desulfoviridin, desulforubidin, P582, and desulfofuscidin were not present. The G+C content of the DNA was 51 mol%. Sequence analysis of 16S rDNA showed that phylogenetically strain A8444 belongs to the delta subdivision of the Proteobacteria. The closest relatives are Desulfacinum infernum and Syntrophobacter wolinii. Strain A8444 is described as the type strain of the new taxon Thermodesulforhabdus norvegicus gen. nov., sp. nov.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050271
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