ISSN:
1089-7623
Source:
AIP Digital Archive
Topics:
Physics
,
Electrical Engineering, Measurement and Control Technology
Notes:
Several years ago a time-resolved circular dichroism technique for the far ultraviolet spectral region with submicrosecond (10−7 s) time resolution was developed using a xenon flash lamp probe source for measurements of circular dichroism (CD) signals. Recent improvements in Ti:sapphire lasers, providing the ability to frequency-convert the fundamental outputs to produce second, third, and fourth harmonic pulses, allow single wavelength measurements of CD with nanosecond (10−9 s) time resolution over a broad spectral region (205–910 nm). This provides a powerful technique to study fast biophysical phenomena such as protein folding processes. In this article, the methodology and preliminary application of this new technique are presented. © 1996 American Institute of Physics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1063/1.1147422
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